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Journal of Virology, July 2009, p. 6929-6933, Vol. 83, No. 13
0022-538X/09/$08.00+0     doi:10.1128/JVI.02299-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Dissecting the Functional Domains of a Nonenveloped Virus Membrane Penetration Peptide{triangledown}

Manidipa Banerjee,1 Reza Khayat,1 Hanna E. Walukiewicz,2,# Amy L. Odegard,1 Anette Schneemann,1 and John E. Johnson1*

Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037,1 Department of Chemistry and Biochemistry, University of California—San Diego, La Jolla, California 920932

Received 3 November 2008/ Accepted 9 April 2009

Recent studies have established that several nonenveloped viruses utilize virus-encoded lytic peptides for host membrane disruption. We investigated this mechanism with the "gamma" peptide of the insect virus Flock House virus (FHV). We demonstrate that the C terminus of gamma is essential for membrane disruption in vitro and the rescue of immature virus infectivity in vivo, and the amphipathic N terminus of gamma alone is not sufficient. We also show that deletion of the C-terminal domain disrupts icosahedral ordering of the amphipathic helices of gamma in the virus. Our results have broad implications for understanding membrane lysis during nonenveloped virus entry.

* Corresponding author. Mailing address: Department of Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-9705. Fax: (858) 784-8660. E-mail: jackj{at}scripps.edu

{triangledown} Published ahead of print on 15 April 2009.

# Present address: Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

Journal of Virology, July 2009, p. 6929-6933, Vol. 83, No. 13
0022-538X/09/$08.00+0     doi:10.1128/JVI.02299-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Odegard, A. L., Kwan, M. H., Walukiewicz, H. E., Banerjee, M., Schneemann, A., Johnson, J. E. (2009). Low Endocytic pH and Capsid Protein Autocleavage Are Critical Components of Flock House Virus Cell Entry. J. Virol. 83: 8628-8637 [Abstract] [Full Text]  


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