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Journal of Virology, June 2009, p. 5943-5946, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00188-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Role of Untranslated Regions of the Hemagglutinin-Neuraminidase Gene in Replication and Pathogenicity of Newcastle Disease Virus{triangledown}

Yongqi Yan, Subrat N. Rout, Shin-Hee Kim, and Siba K. Samal*

Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, Maryland 20742

Received 27 January 2009/ Accepted 18 March 2009

To determine the role of untranslated regions (UTRs) in replication and pathogenesis of Newcastle disease virus (NDV), we generated recombinant viruses with deletions in 5' and 3' UTRs of the HN mRNA. Deletion of any HN UTR did not noticeably affect in vitro replication of these viruses. However, complete deletion of the 5' UTR of the HN gene decreased the HN mRNA levels and HN protein contents in virus particles, resulting in attenuation of the virus in chickens. This indicates that the 5' UTR of HN mRNA plays an important role in replication and pathogenicity of NDV in vivo.

* Corresponding author. Mailing address: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742. Phone: (301) 314-6813. Fax: (301) 314-6855. E-mail: ssamal{at}umd.edu

{triangledown} Published ahead of print on 25 March 2009.

Journal of Virology, June 2009, p. 5943-5946, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00188-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kim, S.-H., Yan, Y., Samal, S. K. (2009). Role of the Cytoplasmic Tail Amino Acid Sequences of Newcastle Disease Virus Hemagglutinin-Neuraminidase Protein in Virion Incorporation, Cell Fusion, and Pathogenicity. J. Virol. 83: 10250-10255 [Abstract] [Full Text]  


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