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Journal of Virology, June 2009, p. 5939-5942, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00180-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Region Required for Protein Expression from the Stop-Start Pentanucleotide in the M Gene of Influenza B Virus{triangledown}

Masato Hatta,1 Candice K. Kohlmeier,1 Yasuko Hatta,1 Makoto Ozawa,1 and Yoshihiro Kawaoka1,2,3*

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, Wisconsin 53706,1 Division of Virology, Department of Microbiology and Immunology and International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan,2 ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama 332-0012, Japan3

Received 26 January 2009/ Accepted 5 March 2009

Segment 7 of influenza B virus encodes two proteins, M1 and BM2. BM2 is expressed from a stop-start pentanucleotide, in which the BM2 initiation codon overlaps with the M1 stop codon. Here, we demonstrate that 45 nucleotides of the 3' end of the M1 coding region, but not the 5' end of the BM2 coding region, are sufficient for the efficient expression of the downstream protein. Placing these 45 nucleotides and the stop-start pentanucleotide in between the coding sequences induced the expression of at least three noninfluenza proteins, suggesting the utility of this system for expressing multiple proteins from one mRNA.

* Corresponding author. Mailing address: Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, WI 53711. Phone: (608) 265-4925. Fax: (608) 265-5622. E-mail: kawaokay{at}svm.vetmed.wisc.edu

{triangledown} Published ahead of print on 11 March 2009.

Journal of Virology, June 2009, p. 5939-5942, Vol. 83, No. 11
0022-538X/09/$08.00+0     doi:10.1128/JVI.00180-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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