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Journal of Virology, May 2009, p. 5046-5055, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.02409-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Andes Virus Antigens Are Shed in Urine of Patients with Acute Hantavirus Cardiopulmonary Syndrome{triangledown} ,{ddagger}

Paula Godoy,1,{dagger} Delphine Marsac,1,{dagger} Elias Stefas,2 Pablo Ferrer,1 Nicole D. Tischler,3 Karla Pino,1 Pablo Ramdohr,1 Pablo Vial,4 Pablo D. T. Valenzuela,3,5 Marcela Ferrés,1 Francisco Veas,6 and Marcelo López-Lastra1*

Laboratorio de Infectología y Virología Molecular, Centro de Investigaciones Médicas y Departamento de Pediatría, Facultad de Medicina, Pontificia Universidad Católica de Chile, Marcoleta 391, Santiago, Chile,1 ApoH Technologies, University Montpellier-1, Faculty of Pharmacy, 15 Ave. Charles Flahault, Building D, 34093 Montpellier, France,2 Fundación Ciencia para la Vida and Instituto MIFAB, Av. Zañartu 1482, Santiago, Chile,3 Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Las Condes 12438, Lo Barnechea, Santiago, Chile,4 Facultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Portugal 49 Santiago, Chile,5 Institut de Recherche pour le Développement, University Montpellier-1, Viral and Molecular Immunology Laboratory, U178/IRD Emerging Diseases, Faculty of Pharmacy, 15 Ave. Charles Flahault, Building D, 34093 Montpellier, France6

Received 21 November 2008/ Accepted 19 February 2009

Hantavirus cardiopulmonary syndrome (HCPS) is a highly pathogenic emerging disease (40% case fatality rate) caused by New World hantaviruses. Hantavirus infections are transmitted to humans mainly by inhalation of virus-contaminated aerosol particles of rodent excreta and secretions. At present, there are no antiviral drugs or immunotherapeutic agents available for the treatment of hantaviral infection, and the survival rates for infected patients hinge largely on early virus recognition and hospital admission and aggressive pulmonary and hemodynamic support. In this study, we show that Andes virus (ANDV) interacts with human apolipoprotein H (ApoH) and that ApoH-coated magnetic beads or ApoH-coated enzyme-linked immunosorbent assay plates can be used to capture and concentrate the virus from complex biological mixtures, such as serum and urine, allowing it to be detected by both immunological and molecular approaches. In addition, we report that ANDV-antigens and infectious virus are shed in urine of HCPS patients.

* Corresponding author. Mailing address: Laboratorio de Virología Molecular, Facultad de Medicina, Pontificia Universidad Católica de Chile, Marcoleta 391, Santiago, Chile. Phone: (56) 2 354 8182. Fax: (56) 2 638 7457. E-mail: malopez{at}med.puc.cl

{triangledown} Published ahead of print on 11 March 2009.

{ddagger} Supplemental material for this article may be found at http://jvi.asm.org/.

{dagger} P.G. and D.M. contributed equally.

Journal of Virology, May 2009, p. 5046-5055, Vol. 83, No. 10
0022-538X/09/$08.00+0     doi:10.1128/JVI.02409-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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