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Journal of Virology, May 2008, p. 4461-4470, Vol. 82, No. 9
0022-538X/08/$08.00+0     doi:10.1128/JVI.01936-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Role of Sindbis Virus Capsid Protein Region II in Nucleocapsid Core Assembly and Encapsidation of Genomic RNA{triangledown}

Ranjit Warrier,1 Benjamin R. Linger,2,{dagger} Barbara L. Golden,2 and Richard J. Kuhn1*

Markey Center for Structural Biology, Department of Biological Sciences, 915 W. State Street,1 Department of Biochemistry, 175 S. University Street, Purdue University, West Lafayette, Indiana 479072

Received 4 September 2007/ Accepted 20 February 2008

Sindbis virus is an enveloped positive-sense RNA virus in the alphavirus genus. The nucleocapsid core contains the genomic RNA surrounded by 240 copies of a single capsid protein. The capsid protein is multifunctional, and its roles include acting as a protease, controlling the specificity of RNA that is encapsidated into nucleocapsid cores, and interacting with viral glycoproteins to promote the budding of mature virus and the release of the genomic RNA into the newly infected cell. The region comprising amino acids 81 to 113 was previously implicated in two processes, the encapsidation of the viral genomic RNA and the stable accumulation of nucleocapsid cores in the cytoplasm of infected cells. In the present study, specific amino acids within this region responsible for the encapsidation of the genomic RNA have been identified. The region that is responsible for nucleocapsid core accumulation has considerable overlap with the region that controls encapsidation specificity.

* Corresponding author. Mailing address: Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, 915 W. State St., West Lafayette, IN 47907. Phone: (765) 494-1164. Fax: (765) 496-1189. E-mail: kuhnr{at}purdue.edu

{triangledown} Published ahead of print on 27 February 2008.

{dagger} Present address: Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, OH.

Journal of Virology, May 2008, p. 4461-4470, Vol. 82, No. 9
0022-538X/08/$08.00+0     doi:10.1128/JVI.01936-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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