This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bertrand, P.
Right arrow Articles by Liu, S.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertrand, P.
Right arrow Articles by Liu, S.-L.

 Previous Article  |  Next Article 

Journal of Virology, March 2008, p. 2555-2559, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.01853-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Jaagsiekte Sheep Retrovirus Utilizes a pH-Dependent Endocytosis Pathway for Entry{triangledown}

Pascale Bertrand,1 Marceline Côté,1 Yi-Min Zheng,1 Lorraine M. Albritton,2 and Shan-Lu Liu1*

Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada,1 Department of Molecular Sciences, University of Tennessee Health Sciences Center, Memphis, Tennessee 381632

Received 22 August 2007/ Accepted 13 December 2007

Using Moloney murine leukemia virus pseudovirions bearing the envelope protein of Jaagsiekte sheep retrovirus (JSRV), we report here that entry was weakly inhibited by lysosomotropic agents but was profoundly blocked by bafilomycin A1 (BafA1). Kinetics studies revealed that JSRV entry is a slow process and was substantially blocked by a dominant-negative mutant of dynamin. Interestingly, a low-pH pulse overcame the BafA1 block to JSRV infection, although this occurred only if virus-bound cells were preincubated at 37°C, consistent with a very early entry event such as endocytosis being required before the low-pH-dependent step occurs. Moreover, JSRV pseudovirions were resistant to low-pH inactivation. Altogether, this study reveals that JSRV utilizes a pH-dependent, dynamin-associated endocytosis pathway for entry that differs from the classical pH-dependent entry pathway of vesicular stomatitis virus.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, McGill University, Montreal, Canada. Phone: (514) 398-4582. Fax: (514) 398-7052. E-mail: shan-lu.liu{at}mcgill.ca

{triangledown} Published ahead of print on 19 December 2007.

Journal of Virology, March 2008, p. 2555-2559, Vol. 82, No. 5
0022-538X/08/$08.00+0     doi:10.1128/JVI.01853-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cote, M., Zheng, Y.-M., Liu, S.-L. (2009). Receptor Binding and Low pH Coactivate Oncogenic Retrovirus Envelope-Mediated Fusion. J. Virol. 83: 11447-11455 [Abstract] [Full Text]  
  • Schowalter, R. M., Chang, A., Robach, J. G., Buchholz, U. J., Dutch, R. E. (2009). Low-pH Triggering of Human Metapneumovirus Fusion: Essential Residues and Importance in Entry. J. Virol. 83: 1511-1522 [Abstract] [Full Text]  
  • Cote, M., Zheng, Y.-M., Albritton, L. M., Liu, S.-L. (2008). Fusogenicity of Jaagsiekte Sheep Retrovirus Envelope Protein Is Dependent on Low pH and Is Enhanced by Cytoplasmic Tail Truncations. J. Virol. 82: 2543-2554 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»