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Journal of Virology, February 2008, p. 1407-1413, Vol. 82, No. 3
0022-538X/08/$08.00+0     doi:10.1128/JVI.01983-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of Two Virus Integration Sites in the Brown Alga Feldmannia Chromosome{triangledown}

Russel H. Meints,1 Richard G. Ivey,2 Amy M. Lee,1 and Tae-Jin Choi3*

Department of Botany and Plant Pathology, Center for Gene Research and Biotechnology, Oregon State University, Corvallis, Oregon,1 Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington,2 Department of Microbiology, Pukyong National University, Busan 608-737, South Korea3

Received 10 September 2007/ Accepted 12 November 2007

Two similar, large double-stranded DNA viruses, Feldmannia species virus 158 (FsV-158) and FsV-178, replicate only in the unilocular reproductive cells (sporangia) of a brown filamentous alga in the genus Feldmannia. Virus particles are not present in vegetative cells but they are produced in the sporangia formed on vegetative filaments that have been transferred newly into culture. Thus, we proposed that these viruses exist in the vegetative cells in a latent form (R. G. Ivey, E. C. Henry, A. M. Lee, L. Klepper, S. K. Krueger, and R. H. Meints, Virology 220:267-273, 1996). In this article we present evidence that the two FsV genomes are integrated into the host genome during vegetative growth. The FsV genome integration sites were identified by cloning the regions where the FsV genome is linked to the host DNA. FsV-158 and FsV-178 are integrated into two distinct locations in the algal genome. In contrast, the integration sites in the two viral genomes are identical. Notably, the integration sites in the host and viruses contain GC and CG dinucleotide sequences, respectively, from which the GC sequences are recovered at both host-virus junctions. The splice sites in the two FsV genomes are predicted to form a stem-loop structure with the CG dinucleotide in the loop portion.

* Corresponding author. Mailing address: Department of Microbiology, Pukyong National University, 599-1, Daeyeon 3-Dong, Nam-Gu, Busan 608-737, South Korea. Phone: 82 51 620 6367. Fax: 82 51 611 6358. E-mail: choitj{at}pknu.ac.kr

{triangledown} Published ahead of print on 21 November 2007.

Journal of Virology, February 2008, p. 1407-1413, Vol. 82, No. 3
0022-538X/08/$08.00+0     doi:10.1128/JVI.01983-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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