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Journal of Virology, December 2008, p. 12580-12584, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01503-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Functional Pseudotyping of Human Immunodeficiency Virus Type 1 Vectors by Western Equine Encephalitis Virus Envelope Glycoprotein {triangledown}

Ananthalakshmi Poluri,1 Rebecca Ainsworth,1 Scott C. Weaver,3 and Richard E. Sutton1,2*

Department of Molecular Virology and Microbiology,1 Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, Texas 77030,2 Department of Pathology, University of Texas Medical Branch, Galveston, Texas 775553

Received 17 July 2008/ Accepted 22 September 2008

We investigated the ability of western equine encephalitis virus envelope glycoproteins (WEEV GP) to pseudotype lentiviral vectors. The titers of WEEV GP-pseudotyped human immunodeficiency virus type 1 (HIV) ranged as high as 8.0 x 104 IU/ml on permissive cells. Sera from WEEV-infected mice specifically neutralized these pseudotypes; cell transduction was also sensitive to changes in pH. The host range of the pseudotyped particles in vitro was somewhat limited, which is atypical for most alphaviruses. HIV vectors pseudotyped by WEEV GP may be a useful tool for characterizing WEEV cell binding and entry and screening for small-molecule inhibitors.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Medicine, Yale University School of Medicine, 300 Cedar St., TAC S161, New Haven, CT 06520. Phone: (203) 737-3648. Fax: (203) 785-6815. E-mail: richard.sutton{at}yale.edu

{triangledown} Published ahead of print on 8 October 2008.

Journal of Virology, December 2008, p. 12580-12584, Vol. 82, No. 24
0022-538X/08/$08.00+0     doi:10.1128/JVI.01503-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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