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Journal of Virology, November 2008, p. 11344-11353, Vol. 82, No. 22
0022-538X/08/$08.00+0     doi:10.1128/JVI.02375-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Structural Evolution of Reoviridae Revealed by Oryzavirus in Acquiring the Second Capsid Shell{triangledown}

Naoyuki Miyazaki,1,2,3 Tamaki Uehara-Ichiki,3 Li Xing,1 Leif Bergman,2 Akifumi Higashiura,4 Atsushi Nakagawa,4 Toshihiro Omura,3* and R. Holland Cheng1,2*

Department of Molecular and Cellular Biology, University of California, Davis, Davis, California 95616,1 Department of Biosciences at Novum, Karolinska Institute, Halsovagen 7, 14157 Huddinge, Sweden,2 National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan,3 Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan4

Received 2 November 2007/ Accepted 3 September 2008

The conservation of the core structure and diversification of the external features among the turreted reoviruses appear to be relevant to structural evolution in facilitating the infection of diverse host species. The structure of Rice ragged stunt virus (RRSV), in the genus Oryzavirus of the family Reoviridae, is determined to show a core composed of capsid shell, clamps, and long turrets. The RRSV core structure is equivalent to the core structure of Orthoreovirus and the virion structure of Cytoplasmic polyhedrosis virus (CPV). In RRSV, five peripheral trimers surround each long turret and sit at the Q trimer position in the T=13l icosahedral symmetry, a structural feature unique to turreted reoviruses. That is, the core of RRSV is partially covered by 60 copies of the peripheral trimer. In contrast, the core of Orthoreovirus is covered by 200 copies of the trimer that sit at the Q, R, S, and T trimer positions. Our results suggest that among the three viruses, RRSV has a structure intermediate between that of Orthoreovirus and the CPV virion. This conclusion coincides with the results of the phylogenetic analysis of amino acid sequences of RNA-dependent RNA polymerases.

* Corresponding author. Mailing address for Toshihiro Omura: National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan. Phone: 81-29-838-8932. Fax: 81-29-838-8929. E-mail: toomura{at}affrc.go.jp. Mailing address for R. Holland Cheng: Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616. E-mail: rhch{at}ucdavis.edu

{triangledown} Published ahead of print on 10 September 2008.

Journal of Virology, November 2008, p. 11344-11353, Vol. 82, No. 22
0022-538X/08/$08.00+0     doi:10.1128/JVI.02375-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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