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 Previous Article

Journal of Virology, November 2008, p. 10959-10962, Vol. 82, No. 21
0022-538X/08/$08.00+0     doi:10.1128/JVI.01085-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Persistent Propagation of Variant Creutzfeldt-Jakob Disease Agent in Murine Spleen Stromal Cell Culture with Features of Mesenchymal Stem Cells{triangledown}

Sergey Akimov, Oksana Yakovleva, Irina Vasilyeva, Carroll McKenzie, and Larisa Cervenakova*

Transmissible Diseases Department, American Red Cross Holland Laboratory, Rockville, Maryland

Received 22 May 2008/ Accepted 31 July 2008

The transmission of variant Creutzfeldt-Jakob disease (vCJD) through blood transfusions has created new concerns about the iatrogenic spread of transmissible spongiform encephalopathies (TSEs)/prion diseases through blood and plasma-derived products and has increased the need to develop efficient methods for detection of the agent in biologics. Here, we report the first successful generation of spleen-derived murine stromal cell cultures that persistently propagate two mouse-adapted isolates of human TSE agents, mouse-adapted vCJD, and Fukuoka 1. These new cell cultures can be used efficiently for studies of the pathogenesis of the disease, for development of diagnostics and therapeutics, and as a rapid ex vivo assay for TSE inactivation/removal procedures.

* Corresponding author. Mailing address: Transmissible Diseases Department, Holland Laboratory, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855. Phone: (301) 738-0765. Fax: (301) 738-0495. E-mail: cervenakl{at}usa.redcross.org

{triangledown} Published ahead of print on 20 August 2008.

Journal of Virology, November 2008, p. 10959-10962, Vol. 82, No. 21
0022-538X/08/$08.00+0     doi:10.1128/JVI.01085-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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