This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guan, W. Articles by Qiu, J. Search for Related Content PubMed PubMed Citation Articles by Guan, W. Articles by Qiu, J.

 Previous Article  |  Next Article 

Journal of Virology, October 2008, p. 9951-9963, Vol. 82, No. 20
0022-538X/08/$08.00+0     doi:10.1128/JVI.01162-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Block to the Production of Full-Length B19 Virus Transcripts by Internal Polyadenylation Is Overcome by Replication of the Viral Genome

Wuxiang Guan,¹ Fang Cheng,¹ Yuko Yoto,^2, Steve Kleiboeker,³ Susan Wong,⁴ Ning Zhi,⁴ David J. Pintel,² and Jianming Qiu^1*

Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas,¹ Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, Columbia, Missouri,² ViraCor Laboratories, Lee's Summit, Missouri 64086,³ Hematology Branch, National Heart, Lung and Blood Institute, Bethesda, Maryland⁴

Received 3 June 2008/ Accepted 28 July 2008

The pre-mRNA processing strategy of the B19 virus is unique among parvoviruses. B19 virus-generated pre-mRNAs are transcribed from a single promoter and are extensively processed by alternative splicing and alternative polyadenylation to generate 12 transcripts. Blockage of the production of full-length B19 virus transcripts at the internal polyadenylation site [(pA)p] was previously reported to be a limiting step in B19 virus permissiveness. We show here that in the absence of genome replication, internal polyadenylation of B19 virus RNAs at (pA)p is favored in cells which are both permissive and nonpermissive for B19 viral replication. Replication of the B19 virus genome, however, introduced either by viral infection or by transfection of an infectious clone into permissive cells or forced by heterologous replication systems in nonpermissive cells, enhanced readthrough of (pA)p and the polyadenylation of B19 virus transcripts at the distal site [(pA)d]. Therefore, replication of the genome facilitates the generation of sufficient full-length transcripts that encode the viral capsid proteins and the essential 11-kDa nonstructural protein. Furthermore, we show that polyadenylation of B19 viral RNA at (pA)p likely competes with splicing at the second intron. Thus, we conclude that replication of the B19 virus genome is the primary limiting step governing B19 virus tropism.

---

* Corresponding author. Mailing address: University of Kansas Medical Center, MS3029, 3901 Rainbow Blvd., Kansas City, KS 66160. Phone: (913) 588-4329. Fax: (913) 588-9275. E-mail: jqiu{at}kumc.edu

Published ahead of print on 6 August 2008.

Present address: Department of Pediatrics, Sapporo Medical University, Sapporo 060-8543, Japan.

---

Journal of Virology, October 2008, p. 9951-9963, Vol. 82, No. 20
0022-538X/08/$08.00+0     doi:10.1128/JVI.01162-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Guan, W., Wong, S., Zhi, N., Qiu, J. (2009). The Genome of Human Parvovirus B19 Can Replicate in Nonpermissive Cells with the Help of Adenovirus Genes and Produces Infectious Virus. J. Virol. 83: 9541-9553 [Abstract] [Full Text]  
• Sun, Y., Chen, A. Y., Cheng, F., Guan, W., Johnson, F. B., Qiu, J. (2009). Molecular Characterization of Infectious Clones of the Minute Virus of Canines Reveals Unique Features of Bocaviruses. J. Virol. 83: 3956-3967 [Abstract] [Full Text]