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Journal of Virology, October 2008, p. 10321-10325, Vol. 82, No. 20
0022-538X/08/$08.00+0     doi:10.1128/JVI.01229-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Monomeric Linear RNA of Citrus Exocortis Viroid Resulting from Processing In Vivo Has 5'-Phosphomonoester and 3'-Hydroxyl Termini: Implications for the RNase and RNA Ligase Involved in Replication{triangledown}

María-Eugenia Gas, Diego Molina-Serrano, Carmen Hernández, Ricardo Flores, and José-Antonio Daròs*

Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Avenida de los Naranjos, 46022 Valencia, Spain

Received 13 June 2008/ Accepted 4 August 2008

Members of the family Pospiviroidae, like Citrus exocortis viroid (CEVd), replicate through an RNA-based asymmetric rolling-circle mechanism in which oligomeric plus-strand [(+)] RNA intermediates are cleaved to monomeric linear (ml) RNA and then circularized. Here we show, by rapid amplification of 5' and 3' cDNA ends and in vitro ligation assays, that ml CEVd (+) RNA resulting from cleavage of a dimeric transcript transgenically expressed in Arabidopsis thaliana contains 5'-phosphomonoester and 3'-hydroxyl termini. The nature of these termini and the double-stranded structure previously proposed as the substrate for cleavage in vivo suggest that a type III RNase catalyzes cleavage and an RNA ligase distinct from tRNA ligase promotes circularization.

* Corresponding author. Mailing address: Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Avenida de los Naranjos, 46022 Valencia, Spain. Phone: 34 963877893. Fax: 34 963877859. E-mail: jadaros{at}ibmcp.upv.es

{triangledown} Published ahead of print on 13 August 2008.

Journal of Virology, October 2008, p. 10321-10325, Vol. 82, No. 20
0022-538X/08/$08.00+0     doi:10.1128/JVI.01229-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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