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Journal of Virology, October 2008, p. 10042-10051, Vol. 82, No. 20
0022-538X/08/$08.00+0     doi:10.1128/JVI.01240-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Bap31 Is a Novel Target of the Human Papillomavirus E5 Protein{triangledown}

Jennifer A. Regan and Laimonis A. Laimins*

Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611

Received 14 June 2008/ Accepted 31 July 2008

The E5 proteins of human papillomaviruses (HPVs) are small hydrophobic proteins that are expressed in the early and late stages of the viral life cycle; however, their role in HPV pathogenesis is not clearly understood. In this study, a split-ubiquitin yeast (Saccharomyces cerevisiae) two-hybrid system was used to identify B-cell-associated protein 31 (Bap31) as a binding partner of HPV E5 proteins. The association of these proteins was confirmed by coimmunoprecipitation of complexes of Bap31 with either HPV type 16 (HPV16) or HPV31 E5. In addition, Bap31 and E5 were found to colocalize in perinuclear patterns consistent with localization to the endoplasmic reticulum. Mutational analysis of E5 identified amino acids in the extreme C terminus as important for stabilizing the interaction with Bap31. Deletion of these C-terminal amino acids of E5 in the context of complete HPV31 genomes resulted in impaired proliferative capacity of HPV-positive keratinocytes following differentiation. When small interfering RNAs were used to reduce the levels of Bap31, the proliferative ability of HPV-positive keratinocytes upon differentiation was also reduced, implicating Bap31 as a regulator of this process. These studies identify a novel binding partner of the high-risk HPV E5 proteins and provide insight into how the E5 proteins may modulate the life cycle in differentiating cells.

* Corresponding author. Mailing address: Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, 320 E. Superior St., Chicago, IL 60611. Phone: (312) 503-0648. Fax: (312) 503-1339. E-mail: l-laimins{at}northwestern.edu

{triangledown} Published ahead of print on 6 August 2008.

Journal of Virology, October 2008, p. 10042-10051, Vol. 82, No. 20
0022-538X/08/$08.00+0     doi:10.1128/JVI.01240-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Condjella, R., Liu, X., Suprynowicz, F., Yuan, H., Sudarshan, S., Dai, Y., Schlegel, R. (2009). The Canine Papillomavirus E5 Protein Signals from the Endoplasmic Reticulum. J. Virol. 83: 12833-12841 [Abstract] [Full Text]  
  • Lazarczyk, M., Cassonnet, P., Pons, C., Jacob, Y., Favre, M. (2009). The EVER Proteins as a Natural Barrier against Papillomaviruses: a New Insight into the Pathogenesis of Human Papillomavirus Infections. Microbiol. Mol. Biol. Rev. 73: 348-370 [Abstract] [Full Text]  


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