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Journal of Virology, August 2008, p. 8051-8058, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.00550-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Structural Insights into Calicivirus Attachment and Uncoating{triangledown} ,{dagger}

David Bhella,1* Derek Gatherer,1 Yasmin Chaudhry,2 Rebecca Pink,1 and Ian G. Goodfellow2

Medical Research Council Virology Unit, Glasgow University, Church St., Glasgow G11 5JR, United Kingdom,1 Department of Virology, Faculty of Medicine, Imperial College London, Norfolk Place, London W2 1PG, United Kingdom2

Received 12 March 2008/ Accepted 22 May 2008

The Caliciviridae family comprises positive-sense RNA viruses of medical and veterinary significance. In humans, caliciviruses are a major cause of acute gastroenteritis, while in animals respiratory illness, conjunctivitis, stomatitis, and hemorrhagic disease are documented. Investigation of virus-host interactions is limited by a lack of culture systems for many viruses in this family. Feline calicivirus (FCV), a member of the Vesivirus genus, provides a tractable model, since it may be propagated in cell culture. Feline junctional adhesion molecule 1 (fJAM-1) was recently identified as a functional receptor for FCV. We have analyzed the structure of this virus-receptor complex by cryo-electron microscopy and three-dimensional image reconstruction, combined with fitting of homology modeled high-resolution coordinates. We show that domain 1 of fJAM-1 binds to the outer face of the P2 domain of the FCV capsid protein VP1, inducing conformational changes in the viral capsid. This study provides the first structural view of a native calicivirus-protein receptor complex and insights into the mechanisms of virus attachment and uncoating.

* Corresponding author. Mailing address: Medical Research Council Virology Unit, Glasgow University, Church St., Glasgow G11 5JR, United Kingdom. Phone: (44) 141-330-3685. Fax: (44) 141-337-2236. E-mail: d.bhella{at}mrcvu.gla.ac.uk

{triangledown} Published ahead of print on 11 June 2008.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, August 2008, p. 8051-8058, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.00550-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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