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Journal of Virology, August 2008, p. 7923-7931, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.00754-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Structural Variations in Species B Adenovirus Fibers Impact CD46 Association {triangledown} ,{dagger}

Lars Pache,1,3 Sangita Venkataraman,2 Vijay S. Reddy,2 and Glen R. Nemerow1*

Department of Immunology and Microbial Science,1 Department of Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037,2 Freie Universität Berlin, Fachbereich Biologie, Chemie, Pharmazie, Takustr. 3, 14195 Berlin, Germany3

Received 4 April 2008/ Accepted 23 May 2008

A majority of species B adenoviruses (Ads) use CD46 as their primary receptor; however, the precise mechanisms involved in the binding of different Ad types to CD46 have not been resolved. Although previous studies indicate close similarities between two members of species B2 Ads in their usage of CD46, our current investigations revealed a surprisingly low CD46 binding affinity of the species B1 Ad16 fiber knob (equilibrium dissociation constant of 437 nM). We determined the crystal structure of the Ad16 fiber knob and constructed a model of this protein in complex with CD46. A comparison of this model to that of the CD46-Ad11 complex revealed structural differences in the FG and IJ loops that are part of the CD46 binding site. An analysis of a panel of recombinant fiber knobs with mutations targeting these regions in Ad16 and Ad11 uncovered a major contribution of the FG loop on CD46 binding. Two extra residues in the FG loop of the Ad16 fiber significantly reduce receptor interaction. Although avidity effects permit the use of CD46 on host cells by Ad16, virus binding occurs with lower efficiency than with B2 Ad types. The longer FG loop of the Ad16 fiber knob also is shared by other species B1 Ad fibers and, thus, may contribute to the low CD46 binding efficiencies observed for these Ad types. Our findings provide a better understanding of how different Ad types associate with CD46 and could aid in the selection of specific Ad fibers for more efficient Ad gene delivery vectors.

* Corresponding author. Mailing address: Department of Immunology and Microbial Science, IMM19, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-8072. Fax: (858) 784-8472. E-mail: gnemerow{at}scripps.edu

{triangledown} Published ahead of print on 4 June 2008.

{dagger} This work is done as manuscript #19477 of The Scripps Research Institute.

Journal of Virology, August 2008, p. 7923-7931, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.00754-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Persson, B. D., Muller, S., Reiter, D. M., Schmitt, B. B. T., Marttila, M., Sumowski, C. V., Schweizer, S., Scheu, U., Ochsenfeld, C., Arnberg, N., Stehle, T. (2009). An Arginine Switch in the Species B Adenovirus Knob Determines High-Affinity Engagement of Cellular Receptor CD46. J. Virol. 83: 673-686 [Abstract] [Full Text]  


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