This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simón, O.
Right arrow Articles by Possee, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simón, O.
Right arrow Articles by Possee, R. D.

 Previous Article  |  Next Article 

Journal of Virology, August 2008, p. 7897-7904, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.00099-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Sf29 Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies{triangledown}

Oihane Simón,1,2,3 Trevor Williams,4 Aaron C. Asensio,2,3 Sarhay Ros,2 Andrea Gaya,2 Primitivo Caballero,2,3 and Robert D. Possee1*

Centre for Ecology and Hydrology, Mansfield Road, Oxford OX1 3SR, United Kingdom,1 Laboratorio de Entomología Agrícola y Patología de Insectos, Universidad Pública de Navarra, 31006 Pamplona, Spain,2 Instituto de Agrobiotecnología, CSIC, Gobierno de Navarra, 31192 Mutilva Baja, Navarra, Spain,3 Instituto de Ecología AC, Apartado Postal 63, Xalapa, Veracruz 91070, México4

Received 15 January 2008/ Accepted 28 May 2008

The genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid (Sfbac) and used to produce a mutant lacking open reading frame 29 (Sf29null). Sf29null bacmid DNA was able to generate an infection in S. frugiperda. Approximately six times less DNA was present in occlusion bodies (OBs) produced by the Sf29null bacmid in comparison to viruses containing this gene. This reduction in DNA content was consistent with fewer virus particles being packaged within Sf29null bacmid OBs, as determined by fractionation of dissolved polyhedra and comparison of occlusion-derived virus (ODV) infectivity in cell culture. DNA from Sfbac, Sf29null, or Sf29null-repair, in which the gene deletion had been repaired, were equally infectious when used to transfect S. frugiperda. All three viruses produced similar numbers of OBs, although those from Sf29null were 10-fold less infectious than viruses with the gene. Insects infected with Sf29null bacmid died ~24 h later than positive controls, consistent with the reduced virus particle content of Sf29null OBs. Transcripts from Sf29 were detected in infected insects 12 h prior to those from the polyhedrin gene. Homologs to Sf29 were present in other group II NPVs, and similar sequences were present in entomopoxviruses. Analysis of the Sf29 predicted protein sequence revealed signal peptide and transmembrane domains, but the presence of 12 potential N-glycosylation sites suggest that it is not an ODV envelope protein. Other motifs, including zinc-binding and threonine-rich regions, suggest degradation and adhesion functions. We conclude that Sf29 is a viral factor that determines the number of ODVs occluded in each OB.

* Corresponding author. Mailing address: Centre for Ecology and Hydrology, Mansfield Road, Oxford OX1 3SR, United Kingdom. Phone: 44 1865 281630. Fax: 44 1865 281696. E-mail: rdpo{at}ceh.ac.uk

{triangledown} Published ahead of print on 11 June 2008.

Journal of Virology, August 2008, p. 7897-7904, Vol. 82, No. 16
0022-538X/08/$08.00+0     doi:10.1128/JVI.00099-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wang, L., Salem, T. Z., Campbell, D. J., Turney, C. M., Kumar, C. M. S., Cheng, X.-W. (2009). Characterization of a virion occlusion-defective Autographa californica multiple nucleopolyhedrovirus mutant lacking the p26, p10 and p74 genes. J. Gen. Virol. 90: 1641-1648 [Abstract] [Full Text]  
  • Yu, I.-L., Bray, D., Lin, Y.-C., Lung, O. (2009). Autographa californica multiple nucleopolyhedrovirus ORF 23 null mutant produces occlusion-derived virions with fewer nucleocapsids. J. Gen. Virol. 90: 1499-1504 [Abstract] [Full Text]  
  • Clavijo, G., Williams, T., Simon, O., Munoz, D., Cerutti, M., Lopez-Ferber, M., Caballero, P. (2009). Mixtures of Complete and pif1- and pif2-Deficient Genotypes Are Required for Increased Potency of an Insect Nucleopolyhedrovirus. J. Virol. 83: 5127-5136 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»