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Journal of Virology, July 2008, p. 6753-6757, Vol. 82, No. 13
0022-538X/08/$08.00+0     doi:10.1128/JVI.00601-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Generation of Recombinant Rotavirus with an Antigenic Mosaic of Cross-Reactive Neutralization Epitopes on VP4{triangledown}

Satoshi Komoto, Masanori Kugita, Jun Sasaki, and Koki Taniguchi*

Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan

Received 18 March 2008/ Accepted 14 April 2008

Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the corresponding sequence of a different P-type DS-1 (human RV strain, G2P[4]). Serological analyses with a panel of anti-VP4- and -VP7-neutralizing monoclonal antibodies revealed that the rescued virus carries a novel antigenic mosaic of cross-reactive neutralization epitopes on its VP4 surface. This is the first report of the generation of a recombinant RV with artificial amino acid substitutions.

* Corresponding author. Mailing address: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan. Phone: 81-562-93-2467. Fax: 81-562-93-4008. E-mail: kokitani{at}fujita-hu.ac.jp

{triangledown} Published ahead of print on 23 April 2008.

Journal of Virology, July 2008, p. 6753-6757, Vol. 82, No. 13
0022-538X/08/$08.00+0     doi:10.1128/JVI.00601-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Desselberger, U., Manktelow, E., Li, W., Cheung, W., Iturriza-Gomara, M., Gray, J. (2009). Rotaviruses and rotavirus vaccines. Br Med Bull 90: 37-51 [Abstract] [Full Text]  


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