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Journal of Virology, July 2008, p. 6747-6752, Vol. 82, No. 13
0022-538X/08/$08.00+0     doi:10.1128/JVI.00247-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Construction and Characterization of a Human T-Cell Lymphotropic Virus Type 3 Infectious Molecular Clone{triangledown}

Sébastien Alain Chevalier,1,4 Nga Ling Ko,1 Sara Calattini,1 Adeline Mallet,2 Marie-Christine Prévost,2 Kylene Kehn,3 John N. Brady,4 Fatah Kashanchi,3 Antoine Gessain,1 and Renaud Mahieux1,3*

Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, CNRS URA 3015, Département de Virologie, Institut Pasteur, 28 rue du Dr. Roux, 75015 Paris, France,1 Plateforme de Microscopie Electronique, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, France,2 Department of Microbiology, Immunology and Tropical Medicine and Department of Biochemistry, The George Washington University Medical Center, Washington, DC 20037,3 NIH/NCI/LCO/VTB, Bethesda, Maryland 208924

Received 4 February 2008/ Accepted 8 April 2008

We and others have uncovered the existence of human T-cell lymphotropic virus type 3 (HTLV-3). We have now generated an HTLV-3 proviral clone. We established that gag, env, pol, pro, and tax/rex as well as minus-strand mRNAs are present in cells transfected with the HTLV-3 clone. HTLV-3 p24gag protein is detected in the cell culture supernatant. Transfection of 293T-long terminal repeat (LTR)-green fluorescent protein (GFP) cells with the HTLV-3 clone promotes formation of syncytia, a hallmark of Env expression, together with the appearance of fluorescent cells, demonstrating that Tax is expressed. Viral particles are visible by electron microscopy. These particles are infectious, as demonstrated by infection experiments with purified virions.

* Corresponding author. Mailing address: INSERM, Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, CNRS URA 3015, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, France. Phone: (33) 1-45-68-89-06. Fax: (33) 1-40-61-34-65. E-mail: rmahieux{at}pasteur.fr

{triangledown} Published ahead of print on 16 April 2008.

Journal of Virology, July 2008, p. 6747-6752, Vol. 82, No. 13
0022-538X/08/$08.00+0     doi:10.1128/JVI.00247-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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