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Journal of Virology, July 2008, p. 6492-6500, Vol. 82, No. 13
0022-538X/08/$08.00+0     doi:10.1128/JVI.00557-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Neutralizing Antibody Blocks Adenovirus Infection by Arresting Microtubule-Dependent Cytoplasmic Transport{triangledown}

Jason G. Smith,1 Aurelia Cassany,2,{dagger} Larry Gerace,2 Robert Ralston,3 and Glen R. Nemerow1*

Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037,1 Department of Cell Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037,2 Department of Virology, Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, New Jersey 070333

Received 12 March 2008/ Accepted 17 April 2008

Neutralizing antibodies are commonly elicited by viral infection. Most antibodies that have been characterized block early stages of virus entry that occur before membrane penetration, whereas inhibition of late stages in entry that occurs after membrane penetration has been poorly characterized. Here we provide evidence that the neutralizing antihexon monoclonal antibody 9C12 inhibits adenovirus infection by blocking microtubule-dependent translocation of the virus to the microtubule-organizing center following endosome penetration. These studies identify a previously undescribed mechanism by which neutralizing antibodies block virus infection, a situation that may be relevant for other nonenveloped viruses that use microtubule-dependent transport during cell entry.

* Corresponding author. Mailing address: 10550 N. Torrey Pines Rd. IMM19, La Jolla, CA 92037. Phone: (858) 784-8072. Fax: (858) 784-8472. E-mail: gnemerow{at}scripps.edu

{triangledown} Published ahead of print on 30 April 2008.

{dagger} Present address: CNRS MCMP UMR 5234, Université Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.

Journal of Virology, July 2008, p. 6492-6500, Vol. 82, No. 13
0022-538X/08/$08.00+0     doi:10.1128/JVI.00557-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Silvestry, M., Lindert, S., Smith, J. G., Maier, O., Wiethoff, C. M., Nemerow, G. R., Stewart, P. L. (2009). Cryo-Electron Microscopy Structure of Adenovirus Type 2 Temperature-Sensitive Mutant 1 Reveals Insight into the Cell Entry Defect. J. Virol. 83: 7375-7383 [Abstract] [Full Text]  
  • Russell, W. C. (2009). Adenoviruses: update on structure and function. J. Gen. Virol. 90: 1-20 [Abstract] [Full Text]  


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