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Journal of Virology, July 2008, p. 6481-6491, Vol. 82, No. 13
0022-538X/08/$08.00+0     doi:10.1128/JVI.00429-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Transcriptional Changes Induced by Bovine Papillomavirus Type 1 in Equine Fibroblasts{triangledown}

Z. Q. Yuan, L. Nicolson, B. Marchetti, E. A. Gault, M. S. Campo, and L. Nasir*

Institute of Comparative Medicine, University of Glasgow Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QH, United Kingdom

Received 27 February 2008/ Accepted 16 April 2008

Bovine papillomavirus type 1 (BPV-1) and, less commonly, BPV-2 are associated with the pathogenesis of common equine skin tumors termed sarcoids. In an attempt to understand the mechanisms by which BPV-1 induces sarcoids, we used gene expression profiling as a screening tool to identify candidate genes implicated in disease pathogenesis. Gene expression profiles of equine fibroblasts transformed by BPV-1 experimentally or from explanted tumors were compared with those of control equine fibroblasts to identify genes associated with expression of BPV-1. Analysis of the microarray data identified 81 probe sets that were significantly (P < 0.01) differentially expressed between the BPV-1-transformed and control cell lines. Expression of several deregulated genes, including MMP-1, CXCL5, FRA-1, NKG7, TLR4, and the gene encoding the major histocompatibility complex class I (MHC-I) protein, was confirmed using other BPV-1-transformed cell lines. Furthermore, expression of these genes was examined using a panel of 10 sarcoids. Increased expression of MMP-1, CXCL5, FRA-1, and NKG7 was detected in a subset of tumors, and TLR4 and MHC I showed robust down-regulation in all tumors. Deregulated expression was confirmed at the protein level for MMP-1 and MHC-I. The present report identifies genes modulated by BPV-1 transformation and will help identify the molecular mechanisms involved in disease pathogenesis.

* Corresponding author. Mailing address: Division of Pathological Sciences, Institute of Comparative Medicine, University of Glasgow, Faculty of Veterinary Medicine, Bearsden Road, Glasgow G61 1QH, Scotland, United Kingdom. Phone: 0141 330 5731. Fax: 0141 330 5602. E-mail: l.nasir{at}vet.gla.ac.uk

{triangledown} Published ahead of print on 23 April 2008.

Journal of Virology, July 2008, p. 6481-6491, Vol. 82, No. 13
0022-538X/08/$08.00+0     doi:10.1128/JVI.00429-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marchetti, B., Gault, E. A., Cortese, M. S., Yuan, Z., Ellis, S. A., Nasir, L., Campo, M. S. (2009). Bovine papillomavirus type 1 oncoprotein E5 inhibits equine MHC class I and interacts with equine MHC I heavy chain. J. Gen. Virol. 90: 2865-2870 [Abstract] [Full Text]  
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