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Journal of Virology, June 2008, p. 6056-6060, Vol. 82, No. 12
0022-538X/08/$08.00+0     doi:10.1128/JVI.02661-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

During Herpes Simplex Virus Type 1 Infection of Rabbits, the Ability To Express the Latency-Associated Transcript Increases Latent-Phase Transcription of Lytic Genes{triangledown}

Nicole V. Giordani,1 Donna M. Neumann,2 Dacia L. Kwiatkowski,1 Partha S. Bhattacharjee,2 Peterjon K. McAnany,1 James M. Hill,2 and David C. Bloom1*

Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida,1 Department of Ophthalmology, Louisiana State University Health Sciences Center, New Orleans, Louisiana2

Received 14 December 2007/ Accepted 27 March 2008

Trigeminal ganglia (TG) from rabbits latently infected with either wild-type herpes simplex virus type 1 (HSV-1) or the latency-associated transcript (LAT) promoter deletion mutant 17{Delta}Pst were assessed for their viral chromatin profile and transcript abundance. The wild-type 17syn+ genomes were more enriched in the transcriptionally permissive mark dimethyl H3 K4 than were the 17{Delta}Pst genomes at the 5' exon and ICP0 and ICP27 promoters. Reverse transcription-PCR analysis revealed significantly more ICP4, tk, and glycoprotein C lytic transcripts in 17syn+ than in 17{Delta}Pst. These results suggest that, for efficient reactivation from latency in rabbits, the LAT is important for increased transcription of lytic genes during latency.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, Box 100266, University of Florida College of Medicine, Gainesville, FL 32610-0266. Phone: (352) 392-8520. Fax: (352) 392-3133. E-mail: dbloom{at}ufl.edu

{triangledown} Published ahead of print on 9 April 2008.

Journal of Virology, June 2008, p. 6056-6060, Vol. 82, No. 12
0022-538X/08/$08.00+0     doi:10.1128/JVI.02661-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Whitlow, Z. W., Kristie, T. M. (2009). Recruitment of the Transcriptional Coactivator HCF-1 to Viral Immediate-Early Promoters during Initiation of Reactivation from Latency of Herpes Simplex Virus Type 1. J. Virol. 83: 9591-9595 [Abstract] [Full Text]  
  • Kwiatkowski, D. L., Thompson, H. W., Bloom, D. C. (2009). The Polycomb Group Protein Bmi1 Binds to the Herpes Simplex Virus 1 Latent Genome and Maintains Repressive Histone Marks during Latency. J. Virol. 83: 8173-8181 [Abstract] [Full Text]  


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