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Journal of Virology, June 2008, p. 6034-6038, Vol. 82, No. 12
0022-538X/08/$08.00+0     doi:10.1128/JVI.00100-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Basolateral Entry and Release of New and Old World Arenaviruses from Human Airway Epithelia{triangledown}

Douglas E. Dylla,1 Daniel E. Michele,2,3,{dagger} Kevin P. Campbell,1,2,3 and Paul B. McCray Jr.1,4*

Genetics Ph.D. Program,1 Howard Hughes Medical Institute,2 Department of Molecular Physiology and Biophysics,3 Department of Pediatrics, The Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 522424

Received 15 January 2008/ Accepted 6 April 2008

Transmission of arenaviruses from rodent hosts to humans is generally thought to occur through inhalation or ingestion of dust or droplets containing viral particles. Here we demonstrate that two identified arenavirus receptors, {alpha}-dystroglycan ({alpha}-DG) and transferrin receptor 1 (TfR1), are expressed in polarized human airway epithelia. Lymphocytic choriomeningitis virus strains with high or low {alpha}-DG affinity and Junin virus, which binds TfR1, efficiently infected polarized epithelia only when applied to the basolateral surface or when injury compromised tight junction integrity. Viral egress from infected epithelia exhibited basolateral polarity. This study demonstrates that respiratory entry of arenaviruses occurs via basolateral receptors.

* Corresponding author. Mailing address: 240F EMRB, Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-6844. Fax: (319) 335-6925. E-mail: paul-mccray{at}uiowa.edu

{triangledown} Published ahead of print on 16 April 2008.

{dagger} Present address: Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, MI 48109.

Journal of Virology, June 2008, p. 6034-6038, Vol. 82, No. 12
0022-538X/08/$08.00+0     doi:10.1128/JVI.00100-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Labudova, M., Tomaskova, J., Skultety, L., Pastorek, J., Pastorekova, S. (2009). The Nucleoprotein of Lymphocytic Choriomeningitis Virus Facilitates Spread of Persistent Infection through Stabilization of the Keratin Network. J. Virol. 83: 7842-7849 [Abstract] [Full Text]  


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