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Journal of Virology, January 2008, p. 291-299, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.01736-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Coupling Genetics and Proteomics To Identify Aphid Proteins Associated with Vector-Specific Transmission of Polerovirus (Luteoviridae){triangledown}

Xiaolong Yang,1,2,3 T. W. Thannhauser,3 Mary Burrows,4 Diana Cox-Foster,2 Fred E. Gildow,1 and Stewart M. Gray4*

Departments of Plant Pathology,1 Entomology, The Pennsylvania State University, University Park, Pennsylvania 16802,2 Plant Protection Research Unit, USDA, ARS, and Department of Plant Pathology, Cornell University, Ithaca, New York 14853,4 Functional and Comparative Proteomics Center, USDA-ARS Plant, Soil, and Nutrition Laboratory, Ithaca, New York 148533

Received 9 August 2007/ Accepted 15 October 2007

Cereal yellow dwarf virus-RPV (CYDV-RPV) is transmitted specifically by the aphids Rhopalosiphum padi and Schizaphis graminum in a circulative nonpropagative manner. The high level of vector specificity results from the vector aphids having the functional components of the receptor-mediated endocytotic pathways to allow virus to transverse the gut and salivary tissues. Studies of F2 progeny from crosses of vector and nonvector genotypes of S. graminum showed that virus transmission efficiency is a heritable trait regulated by multiple genes acting in an additive fashion and that gut- and salivary gland-associated factors are not genetically linked. Utilizing two-dimensional difference gel electrophoresis to compare the proteomes of vector and nonvector parental and F2 genotypes, four aphid proteins (S4, S8, S29, and S405) were specifically associated with the ability of S. graminum to transmit CYDV-RPV. The four proteins were coimmunoprecipitated with purified RPV, indicating that the aphid proteins are capable of binding to virus. Analysis by mass spectrometry identified S4 as a luciferase and S29 as a cyclophilin, both of which have been implicated in macromolecular transport. Proteins S8 and S405 were not identified from available databases. Study of this unique genetic system coupled with proteomic analysis indicated that these four virus-binding aphid proteins were specifically inherited and conserved in different generations of vector genotypes and suggests that they play a major role in regulating polerovirus transmission.

* Corresponding author. Mailing address: Department of Plant Pathology, Cornell University, Ithaca, NY 14853. Phone: (607) 255-7844. Fax: (607) 255-2459. E-mail: smg3{at}cornell.edu

{triangledown} Published ahead of print on 24 October 2007.

Journal of Virology, January 2008, p. 291-299, Vol. 82, No. 1
0022-538X/08/$08.00+0     doi:10.1128/JVI.01736-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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