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Journal of Virology, May 2007, p. 4585-4590, Vol. 81, No. 9
0022-538X/07/$08.00+0     doi:10.1128/JVI.02391-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Proteins Associated with Culex nigripalpus Nucleopolyhedrovirus Occluded Virions{triangledown}

Omaththage Perera,1* Terry B. Green,2 Stanley M. Stevens Jr.,3 Susan White,2 and James J. Becnel2

Southern Insect Management Research Unit, USDA-ARS, 141 Experiment Station Road, P.O. Box 346, Stoneville, Mississippi 38776,1 Center for Medical, Agricultural and Veterinary Entomology, USDA/ARS, 1600-1700 SW 23rd Avenue, Gainesville, Florida 32608,2 Proteomics Core, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, Florida 326103

Received 31 October 2006/ Accepted 2 February 2007

Occlusion-derived virions (ODVs) of the nucleopolyhedrovirus of Culex nigripalpus (CuniNPV) were purified by Ludox density gradient ultracentrifugation, and the proteins were separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Proteins were identified by using Edman sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry, nanoelectrospray quadrupole time-of-flight mass spectrometry, or a combination of these methods. Half of the 44 polypeptide sequences identified in this analysis were unique open reading frames (ORFs) encoded by the CuniNPV genome and did not show similarity to any other sequences present in protein databases. Of the 22 polypeptides that showed similarities to other baculovirus-encoded proteins, only 17 sequences have previously been identified as structural proteins. The newly identified CuniNPV structural proteins cun058, cun059, cun087, cun106, and cun109 are homologues of Autographa californica nucleopolyhedrovirus (AcMNPV) ORFs 68, 62, 98, 81, and 2, respectively. The products of four genes, namely, lef-1 (cun045), alkaline exonuclease (cun054), helicase (cun089), and DNA polymerase (cun091), were not detected in the CuniNPV ODV preparations. These four genes are conserved among all annotated baculovirus genomes, and their homologues have been detected in the ODV of AcMNPV.

* Corresponding author. Mailing address: Southern Insect Management, Research Unit, 141 Experiment Station Road, P.O. Box 346, Stoneville, MS 38776. Phone: (662) 686-5248. Fax: (662) 686-5421. E-mail: operera{at}msa-stoneville.ars.usda.gov

{triangledown} Published ahead of print on 14 February 2007.

Journal of Virology, May 2007, p. 4585-4590, Vol. 81, No. 9
0022-538X/07/$08.00+0     doi:10.1128/JVI.02391-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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