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Journal of Virology, April 2007, p. 3272-3284, Vol. 81, No. 7
0022-538X/07/$08.00+0     doi:10.1128/JVI.01530-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Contrasting Effects of Human, Canine, and Hybrid Adenovirus Vectors on the Phenotypical and Functional Maturation of Human Dendritic Cells: Implications for Clinical Efficacy{triangledown}

Matthieu Perreau,1,2,{dagger} Franck Mennechet,2,{dagger} Nicolas Serratrice,1,2 Joel N. Glasgow,3,{ddagger} David T. Curiel,3,4 Harald Wodrich,1,2 and Eric J. Kremer1,2*

Institut de Génétique Moléculaire de Montpellier,1 CNRS-University of Montpellier II and IFR 122, 34293 Montpellier, France,2 Division of Human Gene Therapy, Department of Medicine,3 Gene Therapy Center, University of Alabama at Birmingham, Birmingham, Alabama4

Received 18 July 2006/ Accepted 29 December 2006

Antipathogen immune responses create a balance between immunity, tolerance, and immune evasion. However, during gene therapy most viral vectors are delivered in substantial doses and are incapable of expressing gene products that reduce the host's ability to detect transduced cells. Gene transfer efficacy is also modified by the in vivo transduction of dendritic cells (DC), which notably increases the immunogenicity of virions and vector-encoded genes. In this study, we evaluated parameters that are relevant to the use of canine adenovirus serotype 2 (CAV-2) vectors in the clinical setting by assaying their effect on human monocyte-derived DC (hMoDC). We compared CAV-2 to human adenovirus (HAd) vectors containing the wild-type virion, functional deletions in the penton base RGD motif, and the CAV-2 fiber knob. In contrast to the HAd type 5 (HAd5)-based vectors, CAV-2 poorly transduced hMoDC, provoked minimal upregulation of major histocompatibility complex class I/II and costimulatory molecules (CD40, CD80, and CD86), and induced negligible morphological changes indicative of DC maturation. Functional maturation assay results (e.g., reduced antigen uptake; tumor necrosis factor alpha, interleukin-1ß [IL-1ß], gamma interferon [IFN-{gamma}], IL-10, IL-12, and IFN-{alpha}/ß secretion; and stimulation of heterologous T-cell proliferation) were also significantly lower for CAV-2. Our data suggested that this was due, in part, to the use of an alternative receptor and a block in vesicular escape. Additionally, HAd5 vector-induced hMoDC maturation was independent of the aforementioned cytokines. Paradoxically, an HAd5/CAV-2 hybrid vector induced the greatest phenotypical and functional maturation of hMoDC. Our data suggest that CAV-2 and the HAd5/CAV-2 vector may be the antithesis of Adenoviridae immunogenicity and that each may have specific clinical advantages.

* Corresponding author. Mailing address: IGMM CNRS 5535, 1919 Route de Mende, 34293 Montpellier, France. Phone: (33) 4 67 61 36 72. Fax: (33) 4 67 04 02 31. E-mail: eric.kremer{at}igmm.cnrs.fr.

{triangledown} Published ahead of print on 17 January 2007.

{dagger} M.P. and F.M. contributed equally to this work.

{ddagger} Present address: Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama.

Journal of Virology, April 2007, p. 3272-3284, Vol. 81, No. 7
0022-538X/07/$08.00+0     doi:10.1128/JVI.01530-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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