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Journal of Virology, March 2007, p. 2995-2998, Vol. 81, No. 6
0022-538X/07/$08.00+0     doi:10.1128/JVI.02486-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Proteolytic Processing of the Ebola Virus Glycoprotein Is Not Critical for Ebola Virus Replication in Nonhuman Primates{triangledown}

Gabriele Neumann,1 Thomas W. Geisbert,2,3 Hideki Ebihara,4,5 Joan B. Geisbert,2 Kathleen M. Daddario-DiCaprio,2,3 Heinz Feldmann,4 and Yoshihiro Kawaoka1,5*

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, Wisconsin,1 United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland,2 Uniformed Services University of the Health Sciences, Bethesda, Maryland,3 Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, and Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada,4 Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan5

Received 12 November 2006/ Accepted 19 December 2006

Enveloped viruses often require cleavage of a surface glycoprotein by a cellular endoprotease such as furin for infectivity and virulence. Previously, we showed that Ebola virus glycoprotein does not require the furin cleavage motif for virus replication in cell culture. Here, we show that there are no appreciable differences in disease progression, hematology, serum biochemistry, virus titers, or lethality in nonhuman primates infected with an Ebola virus lacking the furin recognition sequence compared to those infected with wild-type virus. We conclude that glycoprotein cleavage by subtilisin-like endoproteases is not critical for Ebola virus infectivity and virulence in nonhuman primates.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, 2015 Linden Drive, Madison, WI 53706. Phone: (608) 265-4925. Fax: (608) 265-5622. E-mail: kawaokay{at}svm.vetmed.wisc.edu.

{triangledown} Published ahead of print on 17 January 2007.

Journal of Virology, March 2007, p. 2995-2998, Vol. 81, No. 6
0022-538X/07/$08.00+0     doi:10.1128/JVI.02486-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bergeron, E., Vincent, M. J., Nichol, S. T. (2007). Crimean-Congo Hemorrhagic Fever Virus Glycoprotein Processing by the Endoprotease SKI-1/S1P Is Critical for Virus Infectivity. J. Virol. 81: 13271-13276 [Abstract] [Full Text]  


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