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Journal of Virology, March 2007, p. 2861-2868, Vol. 81, No. 6
0022-538X/07/$08.00+0     doi:10.1128/JVI.02291-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Suppression of the Sendai Virus M Protein through a Novel Short Interfering RNA Approach Inhibits Viral Particle Production but Does Not Affect Viral RNA Synthesis{triangledown}

Geneviève Mottet-Osman,1 Frédéric Iseni,1,3 Thierry Pelet,1 Maciej Wiznerowicz,1,2 Dominique Garcin,1 and Laurent Roux1*

Department of Microbiology and Molecular Medicine, University of Geneva Medical School, CMU, 1 Rue Michel-Servet, CH-1211 Geneva 4, Switzerland,1 Swiss Federal Institute of Technology, School of Life Sciences, CH-1015 Lausanne, Switzerland,2 Centre de Recherche du Service de Santé des Armées, 24 Avenue des Maquis du Grésivaudan, F-38702, La Tronche, France3

Received 19 October 2006/ Accepted 14 December 2006

Short RNA interference is more and more widely recognized as an effective method to specifically suppress viral functions in eukaryotic cells. Here, we used an experimental system that allows suppression of the Sendai virus (SeV) M protein by using a target sequence, derived from the green fluorescent protein gene, that was introduced in the 3' untranslated region of the M protein mRNA. Silencing of the M protein gene was eventually achieved by a small interfering RNA (siRNA) directed against this target sequence. This siRNA was constitutively expressed in a cell line constructed by transduction with an appropriate lentivirus vector. Suppression of the M protein was sufficient to diminish virus production by 50- to 100-fold. This level of suppression had no apparent effect on viral replication and transcription, supporting the lack of M involvement in SeV transcription or replication control.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Medicine, University of Geneva Medical School, CMU, 1 Rue Michel-Servet, CH-1211 Geneva 4, Switzerland. Phone: 41 22 379 5683. Fax: 41 22 379 5702. E-mail: laurent.roux{at}medecine.unige.ch.

{triangledown} Published ahead of print on 27 December 2006.

Journal of Virology, March 2007, p. 2861-2868, Vol. 81, No. 6
0022-538X/07/$08.00+0     doi:10.1128/JVI.02291-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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