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 Previous Article

Journal of Virology, February 2007, p. 2087-2091, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.02011-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Monoclonal Antibodies to Phosphatidylinositol Phosphate Neutralize Human Immunodeficiency Virus Type 1: Role of Phosphate-Binding Subsites{triangledown}

Bruce K. Brown,1 Nicos Karasavvas,2 Zoltan Beck,1 Gary R. Matyas,2 Deborah L. Birx,2,{dagger} Victoria R. Polonis,2 and Carl R. Alving2*

Henry M. Jackson Foundation, 13 Taft Court, Rockville, Maryland 20850,1 U.S. Military HIV Research Program, Division of Retrovirology, Walter Reed Army Institute of Research, 1600 East Gude Drive, Rockville, Maryland 208502

Received 14 September 2006/ Accepted 20 November 2006

Both a murine monoclonal antibody to phosphatidylinositol phosphate (PIP) and a human monoclonal antibody (4E10) that is known to have broadly neutralizing capabilities against primary isolates of human immunodeficiency virus type 1 (HIV-1) bound to PIP, as determined by enzyme-linked immunosorbent assay. Each of the antibodies had antigen subsite binding specificities in aqueous medium for small phosphate-containing molecules and for inositol. The anti-PIP monoclonal antibody inhibited infection by two HIV-1 primary isolates in neutralization assays employing primary human peripheral blood mononuclear cells. The data suggest that PIP or related lipids having free phosphates could serve as targets for the neutralization of HIV-1.

* Corresponding author. Mailing address: Department of Vaccine Production and Delivery, Division of Retrovirology, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, 1600 East Gude Drive, Rockville, MD 20850. Phone: (301) 251-5061. Fax: (301) 762-7460. E-mail: calving{at}hivresearch.org.

{triangledown} Published ahead of print on 6 December 2006.

{dagger} Present address: Global AIDS Program, CDC/NCHSTP, Corporate Square Building 8, Atlanta, GA 30329.

Journal of Virology, February 2007, p. 2087-2091, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.02011-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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