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Journal of Virology, February 2007, p. 2078-2082, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.02525-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Replication and Propagation of Attenuated Vesicular Stomatitis Virus Vectors In Vivo: Vector Spread Correlates with Induction of Immune Responses and Persistence of Genomic RNA{triangledown}

Ian D. Simon,1,2 Jean Publicover,1,2 and John K. Rose2*

Section of Microbial Pathogenesis,1 Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 065102

Received 15 November 2006/ Accepted 16 November 2006

Live-attenuated vesicular stomatitis virus (VSV) vectors expressing foreign antigens induce potent immune responses and protect against viral diseases in animal models. Highly attenuated (VSV-CT1) or single-cycle VSV (VSV{Delta}G) vectors induce immune responses lower than those generated by attenuated wild-type VSV vectors when given intranasally. We show here that reduced spread of the more highly attenuated or single-cycle vectors to other organs, including lymph nodes, correlates with the reduction in the immune responses. A reverse transcription, real-time PCR assay for VSV genomic RNA (gRNA) sequences showed long-term persistence of gRNA from replicating vectors in lymph nodes, long after viral clearance. Such persistence may be important for induction of potent immune responses by VSV vectors.

* Corresponding author. Mailing address: Department of Pathology, Yale University School of Medicine, 310 Cedar Street (LH315), New Haven, CT 06510. Phone: (203) 785-6794. Fax: (203) 785-6127. E-mail: john.rose{at}yale.edu.

{triangledown} Published ahead of print on 6 December 2006.

Journal of Virology, February 2007, p. 2078-2082, Vol. 81, No. 4
0022-538X/07/$08.00+0     doi:10.1128/JVI.02525-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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  • Turner, D. L., Cauley, L. S., Khanna, K. M., Lefrancois, L. (2007). Persistent Antigen Presentation after Acute Vesicular Stomatitis Virus Infection. J. Virol. 81: 2039-2046 [Abstract] [Full Text]  


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