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Journal of Virology, February 2007, p. 1517-1523, Vol. 81, No. 3
0022-538X/07/$08.00+0     doi:10.1128/JVI.01780-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Not All Cytokine-Producing CD8+ T Cells Suppress Simian Immunodeficiency Virus Replication{triangledown}

Chungwon Chung,1 Wonhee Lee,1 John T. Loffredo,1 Benjamin Burwitz,1 Thomas C. Friedrich,1 Juan Pablo Giraldo Vela,2 Gnankang Napoe,1 Eva G. Rakasz,1 Nancy A. Wilson,1 David B. Allison,3 and David I. Watkins1,2*

Wisconsin National Primate Research Center,1 Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, Madison, Wisconsin 53715-1299,2 Department of Biostatistics, University of Alabama at Birmingham, Birmingham, Alabama 352943

Received 16 August 2006/ Accepted 9 November 2006

Current assays of CD8+ T-lymphocyte function measure cytokine production rather than the ability of these lymphocytes to suppress viral replication. Here we show that CD8+ T-cell clones recognizing the same epitope vary enormously in the ability to suppress simian immunodeficiency virus SIVmac239 replication in an in vitro suppression assay. However, all Nef165-173IW9- and Vif66-73HW8-specific clones from elite controllers effectively suppressed SIV replication. Interestingly, in vitro suppression efficacy was not always associated with the ability to produce gamma interferon, tumor necrosis factor alpha, or interleukin-2.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, University of Wisconsin—Madison, 555 Science Dr., Madison, WI 53711. Phone: (608) 265-3380. Fax: (608) 265-8084. E-mail: watkins{at}primate.wisc.edu.

{triangledown} Published ahead of print on 29 November 2006.

Journal of Virology, February 2007, p. 1517-1523, Vol. 81, No. 3
0022-538X/07/$08.00+0     doi:10.1128/JVI.01780-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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