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Journal of Virology, February 2007, p. 1502-1505, Vol. 81, No. 3
0022-538X/07/$08.00+0     doi:10.1128/JVI.01711-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Silencing of both ß-TrCP1 and HOS (ß-TrCP2) Is Required To Suppress Human Immunodeficiency Virus Type 1 Vpu-Mediated CD4 Down-Modulation{triangledown}

Christophe Butticaz,1 Olivier Michielin,2,3,4 Josiane Wyniger,1 Amalio Telenti,1* and Sylvia Rothenberger1*

Institute of Microbiology, University Hospital of Lausanne, Lausanne, Switzerland,1 Multidisciplinary Oncology Center, University Hospital of Lausanne, Lausanne, Switzerland,2 Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland,3 Swiss Institute of Bioinformatics, Epalinges, Switzerland4

Received 8 August 2006/ Accepted 2 November 2006

The human immunodeficiency virus type 1 (HIV-1) Vpu protein interacts with CD4 within the endoplasmic reticula of infected cells and targets CD4 for degradation through interaction with ß-TrCP1. Mammals possess a homologue of ß-TrCP1, HOS, which is also named ß-TrCP2. We show by coimmunoprecipitation experiments that ß-TrCP2 binds Vpu and is able to induce CD4 down-modulation as efficiently as ß-TrCP1. In two different cell lines, HeLa CD4+ and Jurkat, Vpu-mediated CD4 down-modulation could not be reversed through the individual silencing of endogenous ß-TrCP1 or ß-TrCP2 but instead required the two genes to be silenced simultaneously.

* Corresponding author. Mailing address: Institute of Microbiology, University Hospital, Lausanne, Switzerland. Phone: 41 21 314 4049. Fax: 41 21 314 4095. E-mail for Sylvia Rothenberger: Sylvia.Rothenberger-Aubert{at}chuv.ch. E-mail for Amalio Telenti: Amalio.Telenti{at}chuv.ch.

{triangledown} Published ahead of print on 22 November 2006.

Journal of Virology, February 2007, p. 1502-1505, Vol. 81, No. 3
0022-538X/07/$08.00+0     doi:10.1128/JVI.01711-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Douglas, J. L., Viswanathan, K., McCarroll, M. N., Gustin, J. K., Fruh, K., Moses, A. V. (2009). Vpu Directs the Degradation of the Human Immunodeficiency Virus Restriction Factor BST-2/Tetherin via a {beta}TrCP-Dependent Mechanism. J. Virol. 83: 7931-7947 [Abstract] [Full Text]  
  • Salim, A., Ratner, L. (2008). Modulation of {beta}-Catenin and E-Cadherin Interaction by Vpu Increases Human Immunodeficiency Virus Type 1 Particle Release. J. Virol. 82: 3932-3938 [Abstract] [Full Text]  


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