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Journal of Virology, December 2007, p. 13533-13543, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.01669-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Human Papillomavirus Type 16 E7 Oncoprotein Associates with the Centrosomal Component {gamma}-Tubulin{triangledown}

Christine L. Nguyen,1,2 Catherine Eichwald,3 Max L. Nibert,2,3 and Karl Münger1,2*

Channing Laboratories, Brigham and Women's Hospital,1 Committee on Virology,2 Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 021153

Received 31 July 2007/ Accepted 20 September 2007

Expression of a high-risk human papillomavirus (HPV) E7 oncoprotein is sufficient to induce aberrant centrosome duplication in primary human cells. The resulting centrosome-associated mitotic abnormalities have been linked to the development of aneuploidy. HPV type 16 (HPV16) E7 induces supernumerary centrosomes through a mechanism that is at least in part independent of the inactivation of the retinoblastoma tumor suppressor pRb and is dependent on cyclin-dependent kinase 2 activity. Here, we show that HPV16 E7 can concentrate around mitotic spindle poles and that a small pool of HPV16 E7 is associated with centrosome fractions isolated by sucrose density gradient centrifugation. The targeting of HPV16 E7 to the centrosome, however, was not sufficient for centrosome overduplication. Nonetheless, we found that HPV16 E7 can associate with the centrosomal regulator {gamma}-tubulin and that the recruitment of {gamma}-tubulin to the centrosome is altered in HPV16 E7-expressing cells. Since the association of HPV16 E7 with {gamma}-tubulin is independent of pRb, p107, and p130, our results suggest that the association with {gamma}-tubulin contributes to the pRb/p107/p130-independent ability of HPV16 E7 to subvert centrosome homeostasis.

* Corresponding author. Mailing address: Channing Laboratories 861, 181 Longwood Ave., Boston, MA 02115. Phone: (617) 525-4282. Fax: (617) 525-4283. E-mail: kmunger{at}rics.bwh.harvard.edu

{triangledown} Published ahead of print on 3 October 2007.

Journal of Virology, December 2007, p. 13533-13543, Vol. 81, No. 24
0022-538X/07/$08.00+0     doi:10.1128/JVI.01669-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kamil, J. P., Hume, A. J., Jurak, I., Munger, K., Kalejta, R. F., Coen, D. M. (2009). Human papillomavirus 16 E7 inactivator of retinoblastoma family proteins complements human cytomegalovirus lacking UL97 protein kinase. Proc. Natl. Acad. Sci. USA 106: 16823-16828 [Abstract] [Full Text]  
  • Nguyen, C. L., Munger, K. (2009). Human Papillomavirus E7 Protein Deregulates Mitosis via an Association with Nuclear Mitotic Apparatus Protein 1. J. Virol. 83: 1700-1707 [Abstract] [Full Text]  
  • Nguyen, C. L., McLaughlin-Drubin, M. E., Munger, K. (2008). Delocalization of the Microtubule Motor Dynein from Mitotic Spindles by the Human Papillomavirus E7 Oncoprotein Is Not Sufficient for Induction of Multipolar Mitoses. Cancer Res. 68: 8715-8722 [Abstract] [Full Text]  


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