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Journal of Virology, November 2007, p. 12680-12684, Vol. 81, No. 22
0022-538X/07/$08.00+0     doi:10.1128/JVI.00556-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of the DNA Sequence Interacting with Kaposi's Sarcoma-Associated Herpesvirus Viral Interferon Regulatory Factor 1{triangledown}

Junsoo Park,1* Myung-Shin Lee,2 Seung-Min Yoo,2 Kwi Wan Jeong,3 Daeyoup Lee,3 Joonho Choe,3 and Taegun Seo2*

Korea Basic Science Institute, Gwangju 500-757, Korea,1 Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 301-832, Korea,2 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea3

Received 16 March 2007/ Accepted 4 September 2007

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma. The open reading frame (K9) of KSHV encodes viral interferon regulatory factor 1 (vIRF1), which functions as a repressor of interferon-mediated signal transduction. The amino-terminal region of vIRF1 displays significant homology to the DNA-binding domain of cellular interferon regulatory factors, supporting the theory that the protein interacts with specific DNA sequences. Here, we identify the consensus sequence of vIRF1-binding sites from a pool of random oligonucleotides. Moreover, our data show that vIRF1 interacts with the K3:viral dihydrofolate reductase:viral interleukin 6 promoter region in the KSHV genome.

* Corresponding author. Mailing address for Junsoo Park: Korea Basic Science Institute, Gwangju 500-757, Korea. Phone: 82-62-530-0867. Fax: 82-62-530-0519. E-mail: junsoo{at}kaist.ac.kr. Mailing address for Taegun Seo: Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon 301-832, Korea. Phone: 82-42-259-1663. Fax: 82-42-259-1669. E-mail: tseo{at}eulji.ac.kr

{triangledown} Published ahead of print on 12 September 2007.

Journal of Virology, November 2007, p. 12680-12684, Vol. 81, No. 22
0022-538X/07/$08.00+0     doi:10.1128/JVI.00556-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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