This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jin, Y.-H.
Right arrow Articles by Kim, B. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jin, Y.-H.
Right arrow Articles by Kim, B. S.

 Previous Article  |  Next Article 

Journal of Virology, November 2007, p. 11690-11702, Vol. 81, No. 21
0022-538X/07/$08.00+0     doi:10.1128/JVI.01034-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Differential Virus Replication, Cytokine Production, and Antigen-Presenting Function by Microglia from Susceptible and Resistant Mice Infected with Theiler's Virus{triangledown}

Young-Hee Jin, Mani Mohindru,{dagger} Min H. Kang, Alyson C. Fuller,{ddagger} Bongsu Kang, Daniel Gallo, and Byung S. Kim*

Department of Microbiology and Immunology and Institute for Neuroscience, Northwestern University Feinberg Medical School, 303 East Chicago Ave., Chicago, Illinois 60611

Received 11 May 2007/ Accepted 13 August 2007

Infection with Theiler's murine encephalomyelitis virus (TMEV) in the central nervous system (CNS) causes an immune system-mediated demyelinating disease similar to human multiple sclerosis in susceptible but not resistant strains of mice. To understand the underlying mechanisms of differential susceptibility, we analyzed viral replication, cytokine production, and costimulatory molecule expression levels in microglia and macrophages in the CNS of virus-infected resistant C57BL/6 (B6) and susceptible SJL/J (SJL) mice. Our results indicated that message levels of TMEV, tumor necrosis factor alpha, beta interferon, and interleukin-6 were consistently higher in microglia from virus-infected SJL mice than in those from B6 mice. However, the levels of costimulatory molecule expression, as well as the ability to stimulate allogeneic T cells, were significantly lower in TMEV-infected SJL mice than in B6 mice. In addition, microglia from uninfected naïve mice displayed differential viral replication, T-cell stimulation, and cytokine production, similar to those of microglia from infected mice. These results strongly suggest that different levels of intrinsic susceptibility to TMEV infection, cytokine production, and T-cell activation ability by microglia contribute to the levels of viral persistence and antiviral T-cell responses in the CNS, which are critical for the differential susceptibility to TMEV-induced demyelinating disease between SJL and B6 mice.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Northwestern University Feinberg Medical School, 303 E. Chicago Ave., Chicago, IL 60611. Phone: (312) 503-8693. Fax: (312) 503-1339. E-mail: bskim{at}northwestern.edu

{triangledown} Published ahead of print on 22 August 2007.

{dagger} Present address: Credit Suisse, Eleven Madison Avenue, New York, NY 10010.

{ddagger} Present address: Sterne Kessler Goldstein and Fox LLC, Washington, DC.

Journal of Virology, November 2007, p. 11690-11702, Vol. 81, No. 21
0022-538X/07/$08.00+0     doi:10.1128/JVI.01034-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jin, Y.-H., Kang, B., Kim, B. S. (2009). Theiler's Virus Infection Induces a Predominant Pathogenic CD4+ T Cell Response to RNA Polymerase in Susceptible SJL/J Mice. J. Virol. 83: 10981-10992 [Abstract] [Full Text]  
  • Hou, W., Kang, H. S., Kim, B. S. (2009). Th17 cells enhance viral persistence and inhibit T cell cytotoxicity in a model of chronic virus infection. JEM 206: 313-328 [Abstract] [Full Text]  
  • Myoung, J., Bahk, Y. Y., Kang, H. S., Dal Canto, M. C., Kim, B. S. (2008). Anticapsid Immunity Level, Not Viral Persistence Level, Correlates with the Progression of Theiler's Virus-Induced Demyelinating Disease in Viral P1-Transgenic Mice. J. Virol. 82: 5606-5617 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»