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Journal of Virology, September 2007, p. 9451-9460, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00499-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Arenavirus Z-Glycoprotein Association Requires Z Myristoylation but Not Functional RING or Late Domains{triangledown}

Althea A. Capul, Mar Perez, Emily Burke, Stefan Kunz, Michael J. Buchmeier, and Juan C. de la Torre*

Molecular and Integrative Neuroscience Department (MIND), Scripps Research Institute, La Jolla, California 92037

Received 8 March 2007/ Accepted 13 June 2007

Generation of infectious arenavirus-like particles requires the virus RING finger Z protein and surface glycoprotein precursor (GPC) and the correct processing of GPC into GP1, GP2, and a stable signal peptide (SSP). Z is the driving force of arenavirus budding, whereas the GP complex (GPc), consisting of hetero-oligomers of SSP, GP1, and GP2, forms the viral envelope spikes that mediate receptor recognition and cell entry. Based on the roles played by Z and GP in the arenavirus life cycle, we hypothesized that Z and the GPc should interact in a manner required for virion formation. Here, using confocal microscopy and coimmunoprecipitation assays, we provide evidence for subcellular colocalization and biochemical interaction, respectively, of Z and the GPc. Our results from mutation-function analysis reveal that Z myristoylation, but not the Z late (L) or RING domain, is required for Z-GPc interaction. Moreover, Z interacted directly with SSP in the absence of other components of the GPc. We obtained similar results with Z and GPC from the prototypical arenavirus lymphocytic choriomeningitis virus and the hemorrhagic fever arenavirus Lassa fever virus.

* Corresponding author. Mailing address: Scripps Research Institute, 10550 North Torrey Pines Road, IMM-6, La Jolla, CA 92037. Phone: (858) 784-9462. Fax: (858) 784-9981. E-mail: juanct{at}scripps.edu

{triangledown} Published ahead of print on 20 June 2007.

Journal of Virology, September 2007, p. 9451-9460, Vol. 81, No. 17
0022-538X/07/$08.00+0     doi:10.1128/JVI.00499-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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