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Journal of Virology, July 2007, p. 7300-7305, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02406-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Murine Gammaherpesvirus 68 Contains Two Functional Lytic Origins of Replication{triangledown}

Heiko Adler,1* Beatrix Steer,1,2 Klaus Freimüller,1 and Jürgen Haas3

Institute of Molecular Immunology, Clinical Cooperation Group Hematopoietic Cell Transplantation, GSF-National Research Center for Environment and Health, Munich, Germany,1 Department of Medicine III, Ludwig-Maximilians-University Munich, Munich, Germany,2 Max von Pettenkofer-Institute, Ludwig-Maximilians-University Munich, Munich, Germany3

Received 2 November 2006/ Accepted 6 April 2007

A 1.25-kbp DNA fragment from the right side of the genome containing the lytic origin of replication (oriLyt) of murine gammaherpesvirus 68 (MHV-68) has been identified by a plasmid replication assay. Here we show that a mutant MHV-68 with a deletion of an essential part of this oriLyt, generated by using an MHV-68 bacterial artificial chromosome, was only slightly attenuated and still able to replicate but that a mutant containing an additional deletion on the left side of the genome was replication deficient. The newly identified region was sufficient to support plasmid replication, thus providing evidence for a second oriLyt.

* Corresponding author. Mailing address: Institute of Molecular Immunology, Clinical Cooperation Group Hematopoietic Cell Transplantation, GSF-National Research Center for Environment and Health, Marchioninistrasse 25, D-81377 Munich, Germany. Phone: 49 89 7099-327. Fax: 49 89 7099-330. E-mail: h.adler{at}gsf.de

{triangledown} Published ahead of print on 18 April 2007.

Journal of Virology, July 2007, p. 7300-7305, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02406-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Flach, B., Steer, B., Thakur, N. N., Haas, J., Adler, H. (2009). The M10 Locus of Murine Gammaherpesvirus 68 Contributes to both the Lytic and the Latent Phases of Infection. J. Virol. 83: 8163-8172 [Abstract] [Full Text]  


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