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Journal of Virology, July 2007, p. 7297-7299, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02468-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The X Protein of Borna Disease Virus Serves Essential Functions in the Viral Multiplication Cycle{triangledown}

Marion Poenisch,{dagger} Sandra Wille,{dagger} Andreas Ackermann, Peter Staeheli,* and Urs Schneider*

Department of Virology, University of Freiburg, 79104 Freiburg, Germany

Received 9 November 2006/ Accepted 3 April 2007

The X gene of Borna disease virus (BDV) encodes a nonstructural 10-kDa protein that can interact with viral polymerase cofactor P, thus regulating polymerase activity. It remained unknown whether X is essential for virus multiplication. All our attempts to generate mutant BDV with a nonfunctional X gene proved unsuccessful. However, a mutant virus with an inactive X gene was able to replicate in Vero cells if an artificial gene cassette encoding X was inserted at a site near the 5' end of the viral genome. These results indicate that X performs essential viral functions.

* Corresponding author. Mailing address: Department of Virology, University of Freiburg, Hermann-Herder-Strasse 11, D-79104 Freiburg, Germany. Phone: 49-761-203-6579. Fax: 49-761-203-5350. E-mail for Urs Schneider: urs.schneider{at}uniklinik-freiburg.de. E-mail for Peter Staeheli: peter.staeheli{at}uniklinik-freiburg.de

{triangledown} Published ahead of print on 11 April 2007.

{dagger} These two authors contributed equally to this work.

Journal of Virology, July 2007, p. 7297-7299, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02468-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Poenisch, M., Wille, S., Schneider, U., Staeheli, P. (2009). Second-site mutations in Borna disease virus overexpressing viral accessory protein X. J. Gen. Virol. 90: 1932-1936 [Abstract] [Full Text]  
  • Poenisch, M., Burger, N., Staeheli, P., Bauer, G., Schneider, U. (2009). Protein X of Borna Disease Virus Inhibits Apoptosis and Promotes Viral Persistence in the Central Nervous Systems of Newborn-Infected Rats. J. Virol. 83: 4297-4307 [Abstract] [Full Text]  
  • Poenisch, M., Wille, S., Staeheli, P., Schneider, U. (2008). Polymerase Read-Through at the First Transcription Termination Site Contributes to Regulation of Borna Disease Virus Gene Expression. J. Virol. 82: 9537-9545 [Abstract] [Full Text]  
  • Poenisch, M., Staeheli, P., Schneider, U. (2008). Viral accessory protein X stimulates the assembly of functional Borna disease virus polymerase complexes. J. Gen. Virol. 89: 1442-1445 [Abstract] [Full Text]  
  • Ackermann, A., Staeheli, P., Schneider, U. (2007). Adaptation of Borna Disease Virus to New Host Species Attributed to Altered Regulation of Viral Polymerase Activity. J. Virol. 81: 7933-7940 [Abstract] [Full Text]  
  • Schneider, U., Ackermann, A., Staeheli, P. (2007). A Borna Disease Virus Vector for Expression of Foreign Genes in Neurons of Rodents. J. Virol. 81: 7293-7296 [Abstract] [Full Text]  


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