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Journal of Virology, July 2007, p. 7011-7021, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02581-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Multiple Anti-Interferon Actions of the Influenza A Virus NS1 Protein{triangledown}

Georg Kochs,1,{dagger}* Adolfo García-Sastre,2,3 and Luis Martínez-Sobrido2,3,{dagger}*

Department of Virology, University of Freiburg, D-79008 Freiburg, Germany,1 Department of Microbiology,2 Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, New York 100293

Received 22 November 2006/ Accepted 6 April 2007

The replication and pathogenicity of influenza A virus (FLUAV) are controlled in part by the alpha/beta interferon (IFN-{alpha}/ß) system. This virus-host interplay is dependent on the production of IFN-{alpha}/ß and on the capacity of the viral nonstructural protein NS1 to counteract the IFN system. Two different mechanisms have been described for NS1, namely, blocking the activation of IFN regulatory factor 3 (IRF3) and blocking posttranscriptional processing of cellular mRNAs. Here we directly compare the abilities of NS1 gene products from three different human FLUAV (H1N1) strains to counteract the antiviral host response. We found that A/PR/8/34 NS1 has a strong capacity to inhibit IRF3 and activation of the IFN-ß promoter but is unable to suppress expression of other cellular genes. In contrast, the NS1 proteins of A/Tx/36/91 and of A/BM/1/18, the virus that caused the Spanish influenza pandemic, caused suppression of additional cellular gene expression. Thus, these NS1 proteins prevented the establishment of an IFN-induced antiviral state, allowing virus replication even in the presence of IFN. Interestingly, the block in gene expression was dependent on a newly described NS1 domain that is important for interaction with the cleavage and polyadenylation specificity factor (CPSF) component of the cellular pre-mRNA processing machinery but is not functional in A/PR/8/34 NS1. We identified the Phe-103 and Met-106 residues in NS1 as being critical for CPSF binding, together with the previously described C-terminal binding domain. Our results demonstrate the capacity of FLUAV NS1 to suppress the antiviral host defense at multiple levels and the existence of strain-specific differences that may modulate virus pathogenicity.

* Corresponding author. Mailing address for Luis Martínez-Sobrido: Mount Sinai School of Medicine, Microbiology Department, One Gustave L. Levy Place, Box 1124, New York, NY 10029. Phone: (212) 241-5802. Fax: (212) 534-1684. E-mail: Luis.Martinez{at}mssm.edu. Mailing address for Georg Kochs: Abteilung Virologie, Institut Med. Mikrobiol. & Hygiene, Hermann-Herder-Strasse 11, 79104 Freiburg, Germany. Phone: 49-761-2036623. Fax: 49-761-2036562. E-mail: georg.kochs{at}uniklinik-freiburg.de

{triangledown} Published ahead of print on 18 April 2007.

{dagger} G.K. and L.M.-S. contributed equally to this work.

Journal of Virology, July 2007, p. 7011-7021, Vol. 81, No. 13
0022-538X/07/$08.00+0     doi:10.1128/JVI.02581-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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