This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Isomura, H.
Right arrow Articles by Tsurumi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Isomura, H.
Right arrow Articles by Tsurumi, T.

 Previous Article  |  Next Article 

Journal of Virology, June 2007, p. 6197-6206, Vol. 81, No. 12
0022-538X/07/$08.00+0     doi:10.1128/JVI.00089-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Late Promoter of the Human Cytomegalovirus Viral DNA Polymerase Processivity Factor Has an Impact on Delayed Early and Late Viral Gene Products but Not on Viral DNA Synthesis{triangledown}

Hiroki Isomura,1* Mark F. Stinski,2 Ayumi Kudoh,1 Sanae Nakayama,1 Satoko Iwahori,1 Yoshitaka Sato,1 and Tatsuya Tsurumi1

Division of Virology, Aichi Cancer Center Research Institute, 1-1, Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan,1 Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, Iowa 522422

Received 13 January 2007/ Accepted 26 March 2007

Transcription of the DNA polymerase processivity factor gene (UL44) of human cytomegalovirus initiates at three distinct start sites, which are differentially regulated during productive infection. Two of these start sites, the distal and proximal sites, are active at early times, and the middle start site is active at only late times after infection (F. Leach and E. S. Mocarski, J. Virol. 63:1783-1791, 1989). Compared to the wild type, UL44 gene expression was lower for recombinant viruses with the distal or the middle TATA element mutated. The transcripts initiating from the distal or middle start site facilitated late viral gene expression. The level of viral DNA synthesis was affected by mutation of the distal TATA element. In contrast, mutation of the middle TATA element did not affect the level of viral DNA synthesis, but it did affect significantly the level of late viral gene expression. Recombinant viruses with the distal or middle TATA element mutated grew more slowly than the wild type at both low and high multiplicities of infection. Reduced expression of the UL44 gene from the late middle viral promoter correlated with decreased late viral protein expression and decreased viral growth.

* Corresponding author. Mailing address: Division of Virology, Aichi Cancer Center Research Institute, 1-1, Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan. Phone: 81-52-762-6111, ext. 7032. Fax: 81-52-763-5233. E-mail: hisomura{at}aichi-cc.jp

{triangledown} Published ahead of print on 4 April 2007.

Journal of Virology, June 2007, p. 6197-6206, Vol. 81, No. 12
0022-538X/07/$08.00+0     doi:10.1128/JVI.00089-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jiang, C., Komazin-Meredith, G., Tian, W., Coen, D. M., Hwang, C. B. C. (2009). Mutations That Increase DNA Binding by the Processivity Factor of Herpes Simplex Virus Affect Virus Production and DNA Replication Fidelity. J. Virol. 83: 7573-7580 [Abstract] [Full Text]  
  • Komazin-Meredith, G., Mirchev, R., Golan, D. E., van Oijen, A. M., Coen, D. M. (2008). Hopping of a processivity factor on DNA revealed by single-molecule assays of diffusion. Proc. Natl. Acad. Sci. USA 105: 10721-10726 [Abstract] [Full Text]  
  • Kalejta, R. F. (2008). Tegument Proteins of Human Cytomegalovirus. Microbiol. Mol. Biol. Rev. 72: 249-265 [Abstract] [Full Text]  
  • Isomura, H., Stinski, M. F., Kudoh, A., Murata, T., Nakayama, S., Sato, Y., Iwahori, S., Tsurumi, T. (2008). Noncanonical TATA Sequence in the UL44 Late Promoter of Human Cytomegalovirus Is Required for the Accumulation of Late Viral Transcripts. J. Virol. 82: 1638-1646 [Abstract] [Full Text]  
  • Isomura, H., Stinski, M. F., Kudoh, A., Nakayama, S., Murata, T., Sato, Y., Iwahori, S., Tsurumi, T. (2008). A cis Element between the TATA Box and the Transcription Start Site of the Major Immediate-Early Promoter of Human Cytomegalovirus Determines Efficiency of Viral Replication. J. Virol. 82: 849-858 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»