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Journal of Virology, June 2007, p. 6141-6145, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.00037-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Structure of Immature West Nile Virus{triangledown}

Ying Zhang, Bärbel Kaufmann, Paul R. Chipman, Richard J. Kuhn, and Michael G. Rossmann*

Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, Indiana 47907-2054

Received 5 January 2007/ Accepted 8 March 2007

The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.

* Corresponding author. Mailing address: Department of Biological Sciences, Purdue University, 915 W. State St., West Lafayette, IN 47907-2054. Phone: (765) 494-4911. Fax: (765) 496-1189. E-mail: mr{at}purdue.edu

{triangledown} Published ahead of print on 21 March 2007.

Journal of Virology, June 2007, p. 6141-6145, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.00037-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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