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Journal of Virology, June 2007, p. 5850-5863, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.02403-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Distinct Group of Hepacivirus/Pestivirus-Like Internal Ribosomal Entry Sites in Members of Diverse Picornavirus Genera: Evidence for Modular Exchange of Functional Noncoding RNA Elements by Recombination{triangledown} ,{dagger}

Christopher U. T. Hellen* and Sylvain de Breyne

Department of Microbiology and Immunology, State University of New York Downstate Medical Center, Brooklyn, New York 11203

Received 1 November 2006/ Accepted 20 March 2007

The 5' untranslated regions (UTRs) of the RNA genomes of Flaviviridae of the Hepacivirus and Pestivirus genera contain internal ribosomal entry sites (IRESs) that are unrelated to the two principal classes of IRESs of Picornaviridae. The mechanism of translation initiation on hepacivirus/pestivirus (HP) IRESs, which involves factor-independent binding to ribosomal 40S subunits, also differs fundamentally from initiation on these picornavirus IRESs. Ribosomal binding to HP IRESs requires conserved sequences that form a pseudoknot and the adjacent IIId and IIIe domains; analogous elements do not occur in the two principal groups of picornavirus IRESs. Here, comparative sequence analysis was used to identify a subset of picornaviruses from multiple genera that contain 5' UTR sequences with significant similarities to HP IRESs. They are avian encephalomyelitis virus, duck hepatitis virus 1, duck picornavirus, porcine teschovirus, porcine enterovirus 8, Seneca Valley virus, and simian picornavirus. Their 5' UTRs are predicted to form several structures, in some of which the peripheral elements differ from the corresponding HP IRES elements but in which the core pseudoknot, domain IIId, and domain IIIe elements are all closely related. These findings suggest that HP-like IRESs have been exchanged between unrelated virus families by recombination and support the hypothesis that RNA viruses consist of modular coding and noncoding elements that can exchange and evolve independently.

* Corresponding author. Mailing address: Dept. of Microbiology and Immunology, SUNY Downstate Medical Center, 450 Clarkson Avenue, Box 44, Brooklyn, NY 11203. Phone: (718) 270-1034. Fax: (718) 270-2656. E-mail: christopher.hellen{at}downstate.edu

{triangledown} Published ahead of print on 28 March 2007.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, June 2007, p. 5850-5863, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.02403-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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