This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyazaki, M.
Right arrow Articles by Matsuoka, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyazaki, M.
Right arrow Articles by Matsuoka, M.

 Previous Article  |  Next Article 

Journal of Virology, June 2007, p. 5714-5723, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.02511-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Preferential Selection of Human T-Cell Leukemia Virus Type 1 Provirus Lacking the 5' Long Terminal Repeat during Oncogenesis{triangledown}

Maki Miyazaki,1 Jun-Ichirou Yasunaga,1 Yuko Taniguchi,1 Sadahiro Tamiya,2 Tatsutoshi Nakahata,3 and Masao Matsuoka1*

Laboratory of Virus Immunology, Institute for Virus Research,1 Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto 606-8507,3 Department of Hematology and Department of Infectious Diseases, Graduate School of Medicine, Kumamoto University, Kumamoto 860-8556, Japan2

Received 15 November 2006/ Accepted 27 February 2007

In adult T-cell leukemia (ATL) cells, a defective human T-cell leukemia virus type 1 (HTLV-1) provirus lacking the 5' long terminal repeat (LTR), designated type 2 defective provirus, is frequently observed. To investigate the mechanism underlying the generation of the defective provirus, we sequenced HTLV-1 provirus integration sites from cases of ATL. In HTLV-1 proviruses retaining both LTRs, 6-bp repeat sequences were adjacent to the 5' and 3' LTRs. In 8 of 12 cases with type 2 defective provirus, 6-bp repeats were identified at both ends. In five of these cases, a short repeat was bound to CA dinucleotides of the pol and env genes at the 5' end, suggesting that these type 2 defective proviruses were formed before integration. In four cases lacking the 6-bp repeat, short (6- to 26-bp) deletions in the host genome were identified, indicating that these defective proviruses were generated after integration. Quantification indicated frequencies of type 2 defective provirus of less than 3.9% for two carriers, which are much lower than those seen for ATL cases (27.8%). In type 2 defective proviruses, the second exons of the tax, rex, and p30 genes were frequently deleted, leaving Tax unable to activate NF-{kappa}B and CREB pathways. The HTLV-1 bZIP factor gene, located on the minus strand, is expressed in ATL cells with this defective provirus, and its coding sequences are intact, suggesting its significance in oncogenesis.

* Corresponding author. Mailing address: Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, Shogoin Kawara-cho 53, Sakyo-ku, Kyoto 606-8507, Japan. Phone: 81-75-751-4048. Fax: 81-75-751-4049. E-mail: mmatsuok{at}virus.kyoto-u.ac.jp

{triangledown} Published ahead of print on 7 March 2007.

Journal of Virology, June 2007, p. 5714-5723, Vol. 81, No. 11
0022-538X/07/$08.00+0     doi:10.1128/JVI.02511-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Landry, S., Halin, M., Vargas, A., Lemasson, I., Mesnard, J.-M., Barbeau, B. (2009). Upregulation of Human T-Cell Leukemia Virus Type 1 Antisense Transcription by the Viral Tax Protein. J. Virol. 83: 2048-2054 [Abstract] [Full Text]  
  • Kines, K. J., Morales, M. E., Mann, V. H., Gobert, G. N., Brindley, P. J. (2008). Integration of reporter transgenes into Schistosoma mansoni chromosomes mediated by pseudotyped murine leukemia virus. FASEB J. 22: 2936-2948 [Abstract] [Full Text]  
  • Taylor, G P (2007). Molecular aspects of HTLV-I infection and adult T-cell leukaemia/lymphoma. J. Clin. Pathol. 60: 1392-1396 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»