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 Previous Article

Journal of Virology, May 2007, p. 5423-5426, Vol. 81, No. 10
0022-538X/07/$08.00+0     doi:10.1128/JVI.02307-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Severe Acute Respiratory Syndrome Coronavirus Accessory Protein 6 Is a Virion-Associated Protein and Is Released from 6 Protein-Expressing Cells{triangledown}

Cheng Huang,1 C. J. Peters,1,2 and Shinji Makino1*

Departments of Microbiology and Immunology,1 Pathology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555-10192

Received 20 October 2006/ Accepted 23 February 2007

Analysis of severe acute respiratory syndrome coronavirus (SCoV) by either sucrose gradient equilibrium centrifugation or a virus capture assay using an anti-SCoV S protein antibody demonstrated that the SCoV 6 protein, which is one of the accessory proteins of SCoV, was incorporated into virus particles. Coexpression of the SCoV S, M, E, and 6 proteins was sufficient for incorporation of the 6 protein into virus-like particles. Cells transfected with plasmid expressing the 6 protein released SCoV 6 protein; however, infected cells released SCoV 6 protein only in association with SCoV particles.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019. Phone: (409) 772-2323. Fax: (409) 772-5065. E-mail: shmakino{at}utmb.edu

{triangledown} Published ahead of print on 7 March 2007.

Journal of Virology, May 2007, p. 5423-5426, Vol. 81, No. 10
0022-538X/07/$08.00+0     doi:10.1128/JVI.02307-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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