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Journal of Virology, May 2007, p. 5132-5143, Vol. 81, No. 10
0022-538X/07/$08.00+0     doi:10.1128/JVI.02799-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Complement Contributes to Inflammatory Tissue Destruction in a Mouse Model of Ross River Virus-Induced Disease{triangledown}

Thomas E. Morrison,1,2,3 Robert J. Fraser,4 Paul N. Smith,5,6 Suresh Mahalingam,4 and Mark T. Heise1,2,3*

Department of Genetics,1 Department of Microbiology and Immunology,2 Carolina Vaccine Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599,3 Centre for Virus Research, University of Canberra, Canberra, ACT 2601, Australia,4 Orthopedic Unit, John James Hospital, Canberra, ACT 2601, Australia,5 The Australian National University Medical School, Canberra, ACT 0200, Australia6

Received 19 December 2006/ Accepted 14 February 2007

Arthritogenic alphaviruses, including Ross River virus (RRV) and chikungunya virus, are mosquito-borne viruses that cause significant human disease worldwide, including explosive epidemics that can result in thousands to millions of infected individuals. Similar to infection of humans, infection of C57BL/6 mice with RRV results in severe monocytic inflammation of bone, joint, and skeletal muscle tissues. We demonstrate here that the complement system, an important component of the innate immune response, enhances the severity of RRV-induced disease in mice. Complement activation products were detected in the inflamed tissues and in the serum of RRV-infected wild-type mice. Furthermore, mice deficient in C3 (C3–/–), the central component of the complement system, developed much less severe disease signs than did wild-type mice. Complement-mediated chemotaxis is essential for many inflammatory arthritides; however, RRV-infected wild-type and C3–/– mice had similar numbers and composition of inflammatory infiltrates within hind limb skeletal muscle tissue. Despite similar inflammatory infiltrates, RRV-infected C3–/– mice exhibited far less severe destruction of skeletal muscle tissue. In addition to these studies, complement activation was also detected in synovial fluid from RRV-infected patients. Taken together, these findings indicate that complement activation occurs in the tissues of humans and mice infected with RRV and suggest that complement plays an essential role in the effector phase, but not the inductive phase, of RRV-induced arthritis and myositis.

* Corresponding author. Mailing address: The Carolina Vaccine Institute, University of North Carolina at Chapel Hill, CB #7292, Chapel Hill, NC 27599. Phone: (919) 843-1492. Fax: (919) 843-6924. E-mail: heisem{at}med.unc.edu

{triangledown} Published ahead of print on 21 February 2007.

Journal of Virology, May 2007, p. 5132-5143, Vol. 81, No. 10
0022-538X/07/$08.00+0     doi:10.1128/JVI.02799-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Johnson, J. B., Grant, K., Parks, G. D. (2009). The Paramyxoviruses Simian Virus 5 and Mumps Virus Recruit Host Cell CD46 To Evade Complement-Mediated Neutralization. J. Virol. 83: 7602-7611 [Abstract] [Full Text]  
  • Morrison, T. E., Simmons, J. D., Heise, M. T. (2008). Complement Receptor 3 Promotes Severe Ross River Virus-Induced Disease. J. Virol. 82: 11263-11272 [Abstract] [Full Text]  
  • Ziegler, S. A., Lu, L., da Rosa, A. P. A. T., Xiao, S.-Y., Tesh, R. B. (2008). An Animal Model for Studying the Pathogenesis of Chikungunya Virus Infection. Am J Trop Med Hyg 79: 133-139 [Abstract] [Full Text]  
  • Weissmann, G. (2008). Gore's Fever and Dante's Inferno: Chikungunya Reaches Ravenna. FASEB J. 22: 635-638 [Full Text]  


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