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Journal of Virology, January 2007, p. 166-172, Vol. 81, No. 1
0022-538X/07/$08.00+0     doi:10.1128/JVI.01953-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Wild-Type Levels of Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Cellular Emerin Protein{triangledown}

Ming-Chieh Shun, Janet E. Daigle, Nick Vandegraaff, and Alan Engelman*

Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, and Division of AIDS, Harvard Medical School, Boston, Massachusetts

Received 7 September 2006/ Accepted 4 October 2006

Preintegration complexes (PICs) mediate retroviral integration, and recent results indicate an important role for the inner nuclear membrane protein emerin in orienting human immunodeficiency virus type 1 (HIV-1) PICs to chromatin for integration. Two other host cell proteins, the barrier-to-autointegration factor (BAF) and lamina-associated polypeptide 2{alpha} (LAP2{alpha}), seemed to play a similar preintegrative role for Moloney murine leukemia virus (MMLV) in addition to HIV-1. In contrast, we determined efficient HIV-1 and MMLV infection of HeLa-P4 cells following potent down-regulation of emerin, BAF, or LAP2{alpha} protein by using short interfering RNA. Mouse embryo fibroblasts ablated for emerin protein through gene knockout support the same level of HIV-1 infection as cells derived from wild-type littermate control animals. As the expression of human emerin in mouse knockout cells fails to affect the level of infectivity achieved in its absence, we conclude that HIV-1 efficiently infects cells in the absence of emerin protein and, by extension, that emerin is not a universally important regulator of HIV-1 infectivity.

* Corresponding author. Mailing address: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, 44 Binney St., Boston, MA 02115. Phone: (617) 632-4361. Fax: (617) 632-3113. E-mail: alan_engelman{at}dfci.harvard.edu.

{triangledown} Published ahead of print on 11 October 2006.

Journal of Virology, January 2007, p. 166-172, Vol. 81, No. 1
0022-538X/07/$08.00+0     doi:10.1128/JVI.01953-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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