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Journal of Virology, May 2006, p. 4363-4371, Vol. 80, No. 9
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.9.4363-4371.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cellular Topoisomerase I Modulates Origin Binding by Bovine Papillomavirus Type 1 E1

Yan Hu, Randolph V. Clower, and Thomas Melendy*

Department of Microbiology and Immunology and Department of Biochemistry, University at Buffalo, and the Witebsky Center for Microbial Pathogenesis and Immunology, 210 Biomedical Research Building, School of Medicine and Biomedical Sciences, Buffalo, New York 14214

Received 22 December 2005/ Accepted 6 February 2006

In addition to viral proteins E1 and E2, bovine papillomavirus type 1 (BPV1) depends heavily on host replication machinery for genome duplication. It was previously shown that E1 binds to and recruits cellular replication proteins to the BPV1 origin of replication, including DNA polymerase {alpha}-primase, replication protein A (RPA), and more recently, human topoisomerase I (Topo I). Here, we show that Topo I specifically stimulates the origin binding of E1 severalfold but has no effect on nonorigin DNA binding. This is highly specific, as binding to nonorigin DNA is not stimulated, and other cellular proteins that bind E1, such as RPA and polymerase {alpha}-primase, show no such effect. The stimulation of E1's origin binding by Topo I is not synergistic with the stimulation by E2. Although the enhanced origin binding of E1 by Topo I requires ATP and Mg2+ for optimal efficiency, ATP hydrolysis is not required. Using an enzyme-linked immunosorbent assay, we showed that the interaction between E1 and Topo I is decreased in the presence of DNA. Our results suggest that Topo I participates in the initiation of papillomavirus DNA replication by enhancing E1 binding to the BPV1 origin.

* Corresponding author. Mailing address: University at Buffalo, Department of Microbiology and Immunology, The School of Medicine and Biomedical Sciences, 213 Biomedical Research Building, 3435 Main Street, Buffalo, NY 14214. Phone: (716) 829-3789. Fax: (716) 829-2158. E-mail: TMelendy{at}buffalo.edu.

Journal of Virology, May 2006, p. 4363-4371, Vol. 80, No. 9
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.9.4363-4371.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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