This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Knebel-Mörsdorf, D.
Right arrow Articles by Guarino, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Knebel-Mörsdorf, D.
Right arrow Articles by Guarino, L. A.

 Previous Article  |  Next Article 

Journal of Virology, April 2006, p. 4168-4173, Vol. 80, No. 8
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.8.4168-4173.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Expression of Baculovirus Late and Very Late Genes Depends on LEF-4, a Component of the Viral RNA Polymerase Whose Guanyltransferase Function Is Essential

Dagmar Knebel-Mörsdorf,3,4 Ilja Quadt,4 Yi Li,2 Laura Montier,2 and Linda A. Guarino1,2*

Departments of Biochemistry and Biophysics,1 Entomology, Texas A&M University, College Station, Texas 77843-2128,2 Department of Neurology,3 Max-Planck-Institute for Neurological Research, University of Cologne, D-50931 Cologne, Germany4

Received 27 December 2005/ Accepted 25 January 2006

Baculovirus lef-4 encodes one subunit of the viral RNA polymerase. Here, we demonstrate the essential nature of LEF-4 by RNA interference and bacmid knockout technology. Silencing of LEF-4 in wild-type virus-infected cells suppressed expression of structural genes, while early expression was unaffected, demonstrating its essential role in late gene expression. After transfection of insect cells with lef-4 mutant bacmid, no viral progeny was produced, further defining its central role in infection. Cotransfection with wild-type lef-4 plasmid restored normal replication, but plasmid encoding a guanyltransferase-deficient version failed to rescue. These results emphasize the importance of the mRNA capping function of LEF-4.

* Corresponding author. Mailing address: Department of Biochemistry, Texas A&M University, 2128 TAMU, College Station, TX 77843-2128. Phone: (979) 845-7556. Fax: (979) 845-9274. E-mail: lguarino{at}tamu.edu.

Journal of Virology, April 2006, p. 4168-4173, Vol. 80, No. 8
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.8.4168-4173.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, Y., Guarino, L. A. (2008). Roles of LEF-4 and PTP/BVP RNA Triphosphatases in Processing of Baculovirus Late mRNAs. J. Virol. 82: 5573-5583 [Abstract] [Full Text]  
  • Quadt, I., van Lent, J. W. M., Knebel-Morsdorf, D. (2007). Studies of the Silencing of Baculovirus DNA Binding Protein. J. Virol. 81: 6122-6127 [Abstract] [Full Text]  
  • Wang, Y., Kleespies, R. G., Huger, A. M., Jehle, J. A. (2007). The Genome of Gryllus bimaculatus Nudivirus Indicates an Ancient Diversification of Baculovirus-Related Nonoccluded Nudiviruses of Insects. J. Virol. 81: 5395-5406 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»