This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wurtzer, S.
Right arrow Articles by Clavel, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wurtzer, S.
Right arrow Articles by Clavel, F.

 Previous Article  |  Next Article 

Journal of Virology, April 2006, p. 3679-3683, Vol. 80, No. 7
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.7.3679-3683.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Functional Central Polypurine Tract Provides Downstream Protection of the Human Immunodeficiency Virus Type 1 Genome from Editing by APOBEC3G and APOBEC3B

Sebastien Wurtzer,{dagger} Armelle Goubard,{dagger} Fabrizio Mammano, Sentob Saragosti, Denise Lecossier, Allan J. Hance, and François Clavel*

Unité de Recherche Antivirale, INSERM U552, and Faculté de Médecine, Université Paris 7 Denis Diderot, Paris F-75018, France

Received 22 August 2005/ Accepted 10 January 2006

Lentiviruses utilize two polypurine tracts for initiation of plus-strand viral DNA synthesis. We have examined to what extent human immunodeficiency virus type 1 plus-strand initiation at the central polypurine tract (cPPT) could protect the viral genome from DNA editing by APOBEC3G and APOBEC3B. The presence of a functional cPPT, but not of a mutated cPPT, extensively reduced editing by both APOBEC3G and APOBEC3B of sequences downstream, but not upstream, of the cPPT, with significant protection observed as far as 400 bp downstream. Thus, in addition to other potential functions, the cPPT could help protect lentiviruses from editing by cytidine deaminases of the APOBEC family.

* Corresponding author. Mailing address: INSERM U552, IMEA, Hôpital Bichat Claude Bernard, 46 rue Henri Huchard, 75018 Paris, France. Phone: 331 4025 6363. Fax: 331 4025 6351. E-mail: clavel{at}bichat.inserm.fr.

{dagger} S.W. and A.G. contributed equally to this study.

Journal of Virology, April 2006, p. 3679-3683, Vol. 80, No. 7
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.7.3679-3683.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Armitage, A. E., Katzourakis, A., de Oliveira, T., Welch, J. J., Belshaw, R., Bishop, K. N., Kramer, B., McMichael, A. J., Rambaut, A., Iversen, A. K. N. (2008). Conserved Footprints of APOBEC3G on Hypermutated Human Immunodeficiency Virus Type 1 and Human Endogenous Retrovirus HERV-K(HML2) Sequences. J. Virol. 82: 8743-8761 [Abstract] [Full Text]  
  • Garfinkel, D. J., Stefanisko, K. M., Nyswaner, K. M., Moore, S. P., Oh, J., Hughes, S. H. (2006). Retrotransposon Suicide: Formation of Ty1 Circles and Autointegration via a Central DNA Flap. J. Virol. 80: 11920-11934 [Abstract] [Full Text]  
  • Suspene, R., Rusniok, C., Vartanian, J.-P., Wain-Hobson, S. (2006). Twin gradients in APOBEC3 edited HIV-1 DNA reflect the dynamics of lentiviral replication. Nucleic Acids Res 34: 4677-4684 [Abstract] [Full Text]  
  • Pace, C., Keller, J., Nolan, D., James, I., Gaudieri, S., Moore, C., Mallal, S. (2006). Population Level Analysis of Human Immunodeficiency Virus Type 1 Hypermutation and Its Relationship with APOBEC3G and vif Genetic Variation.. J. Virol. 80: 9259-9269 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»