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Journal of Virology, March 2006, p. 3088-3091, Vol. 80, No. 6
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.6.3088-3091.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Sequential CD134-CXCR4 Interactions in Feline Immunodeficiency Virus (FIV): Soluble CD134 Activates FIV Env for CXCR4-Dependent Entry and Reveals a Cryptic Neutralization Epitope

Aymeric de Parseval,1* Chris K. Grant,2 K. Jagannadha Sastry,3 and John H. Elder1*

The Scripps Research Institute, Department of Molecular Biology, La Jolla, California 92037,1 Custom Monoclonals, Inc., West Sacramento, California,2 The University of Texas M. D. Anderson Cancer Center, Department of Immunology, Houston, Texas 770303

Received 26 October 2005/ Accepted 9 December 2005

Recombinant soluble CD134 (sCD134) facilitated feline immunodeficiency virus (FIV) entry into CXCR4-positive, cell surface CD134-negative target cells. sCD134-activated entry was dose dependent and CXCR4 dependent. We used the sCD134 activation system to explore the neutralization by four anti-V3 monoclonal antibodies (MAbs). V3 MAbs weakly neutralized FIV infection using target cells expressing both CD134 and CXCR4 but potently inhibited sCD134-activated entry into target cells expressing CXCR4 alone. These findings provide direct evidence for a sequential interaction of FIV Env with CD134 and CXCR4 and reveal the presence of a cryptic epitope in V3 that is masked in the mature envelope oligomers.

* Corresponding author. Mailing address: Department of Molecular Biology, MB-14, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone for Aymeric de Parseval: (858) 784-2932. Fax: (858) 784-2750. E-mail: parseval{at}scripps.edu. Phone for John H. Elder: (858) 784-8270. Fax: (858) 784-2750. E-mail: jelder{at}scripps.edu.

Journal of Virology, March 2006, p. 3088-3091, Vol. 80, No. 6
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.6.3088-3091.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sundstrom, M., White, R. L., de Parseval, A., Sastry, K. J., Morris, G., Grant, C. K., Elder, J. H. (2008). Mapping of the CXCR4 Binding Site within Variable Region 3 of the Feline Immunodeficiency Virus Surface Glycoprotein. J. Virol. 82: 9134-9142 [Abstract] [Full Text]  
  • Reggeti, F., Ackerley, C., Bienzle, D. (2008). CD134 and CXCR4 expression corresponds to feline immunodeficiency virus infection of lymphocytes, macrophages and dendritic cells. J. Gen. Virol. 89: 277-287 [Abstract] [Full Text]  
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