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Journal of Virology, March 2006, p. 2808-2814, Vol. 80, No. 6
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.6.2808-2814.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Crystal Structure of the Oligomerization Domain of the Phosphoprotein of Vesicular Stomatitis Virus

Haitao Ding, Todd J. Green, Shanyun Lu, and Ming Luo*

Department of Microbiology, School of Medicine, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, Alabama 35294

Received 29 September 2005/ Accepted 13 December 2005

In the replication cycle of nonsegmented negative-strand RNA viruses, the viral RNA-dependent RNA polymerase (L) recognizes a nucleoprotein (N)-enwrapped RNA template during the RNA polymerase reaction. The viral phosphoprotein (P) is a polymerase cofactor essential for this recognition. We report here the 2.3-Å-resolution crystal structure of the central domain (residues 107 to 177) of P from vesicular stomatitis virus. The fold of this domain consists of a ß hairpin, an {alpha} helix, and another ß hairpin. The {alpha} helix provides the stabilizing force for forming a homodimer, while the two ß hairpins add additional stabilization by forming a four-stranded ß sheet through domain swapping between two molecules. This central dimer positions the N- and C-terminal domains of P to interact with the N and L proteins, allowing the L protein to specifically recognize the nucleocapsid-RNA template and to progress along the template while concomitantly assembling N with nascent RNA. The interdimer interactions observed in the noncrystallographic packing may offer insight into the mechanism of the RNA polymerase processive reaction along the viral nucleocapsid-RNA template.

* Corresponding author. Mailing address: Room 111, CBSE, 1025 18th Street South, University of Alabama at Birmingham, Birmingham, AL 35294. Phone: (205) 934-4259. Fax: (205) 975-9578. E-mail: mingluo{at}uab.edu.

Journal of Virology, March 2006, p. 2808-2814, Vol. 80, No. 6
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.6.2808-2814.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, X., Green, T. J., Tsao, J., Qiu, S., Luo, M. (2008). Role of Intermolecular Interactions of Vesicular Stomatitis Virus Nucleoprotein in RNA Encapsidation. J. Virol. 82: 674-682 [Abstract] [Full Text]  
  • Chen, M., Ogino, T., Banerjee, A. K. (2007). Interaction of Vesicular Stomatitis Virus P and N Proteins: Identification of Two Overlapping Domains at the N Terminus of P That Are Involved in N0-P Complex Formation and Encapsidation of Viral Genome RNA. J. Virol. 81: 13478-13485 [Abstract] [Full Text]  


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